TY - JOUR
T1 - Predominance of modern Mycobacterium tuberculosis strains and active transmission of Beijing sublineage in Jayapura, Indonesia Papua
AU - Chaidir, Lidya
AU - Sengstake, Sarah
AU - de Beer, Jessica
AU - Oktavian, Antonius
AU - Krismawati, Hana
AU - Muhapril, Erfin
AU - Kusumadewi, Inri
AU - Annisa, Jessi
AU - Anthony, Richard
AU - Van Soolingen, Dick
AU - Achmad, Tri Hanggono
AU - Marzuki, Sangkot
AU - Alisjahbana, Bachti
AU - van Crevel, Reinout
N1 - NPP
PY - 2016
Y1 - 2016
N2 - Mycobacterium tuberculosis genotype distribution is different between West and Central Indonesia, but there are no data on the most Eastern part, Papua. We aimed to identify the predominant genotypes of M. tuberculosis responsible for tuberculosis in coastal Papua, their transmission, and the association with patient characteristics. A total of 199 M. tuberculosis isolates were collected. Spoligotyping was applied to describe the population structure of M. tuberculosis, lineage identification was performed using a combination of lineage-specific markers, and genotypic clusters were identified using a combination of 24-locus-MIRU-VNTR and spoligotyping. A high degree of genetic diversity was observed among isolates based on their spoligopatterns. Strains from modern lineage 4 made up almost half of strains (46.9%), being more abundant than the ancient lineage 1 (33.7%), and modern lineage 2 (19.4%). Thirty-five percent of strains belonged to genotypic clusters, especially strains in the Beijing genotype. Previous TB treatment and mutations associated with drug resistance were more common in patients infected with strains of the Beijing genotype. Papua shows a different distribution of M. tuberculosis genotypes compared to other parts of Indonesia. Clustering and drug resistance of modern strains recently introduced to Papua may contribute to the high tuberculosis burden in this region.
AB - Mycobacterium tuberculosis genotype distribution is different between West and Central Indonesia, but there are no data on the most Eastern part, Papua. We aimed to identify the predominant genotypes of M. tuberculosis responsible for tuberculosis in coastal Papua, their transmission, and the association with patient characteristics. A total of 199 M. tuberculosis isolates were collected. Spoligotyping was applied to describe the population structure of M. tuberculosis, lineage identification was performed using a combination of lineage-specific markers, and genotypic clusters were identified using a combination of 24-locus-MIRU-VNTR and spoligotyping. A high degree of genetic diversity was observed among isolates based on their spoligopatterns. Strains from modern lineage 4 made up almost half of strains (46.9%), being more abundant than the ancient lineage 1 (33.7%), and modern lineage 2 (19.4%). Thirty-five percent of strains belonged to genotypic clusters, especially strains in the Beijing genotype. Previous TB treatment and mutations associated with drug resistance were more common in patients infected with strains of the Beijing genotype. Papua shows a different distribution of M. tuberculosis genotypes compared to other parts of Indonesia. Clustering and drug resistance of modern strains recently introduced to Papua may contribute to the high tuberculosis burden in this region.
KW - Adult
KW - Evolution, Molecular
KW - Female
KW - Genetic Variation
KW - Genotype
KW - Humans
KW - Indonesia/epidemiology
KW - Male
KW - Minisatellite Repeats
KW - Molecular Typing
KW - Mycobacterium tuberculosis/classification
KW - Phylogeny
KW - Tuberculosis/epidemiology
KW - Young Adult
U2 - 10.1016/j.meegid.2016.01.019
DO - 10.1016/j.meegid.2016.01.019
M3 - A1: Web of Science-article
C2 - 26825253
SN - 1567-1348
VL - 39
SP - 187
EP - 193
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -