Predominance of modern Mycobacterium tuberculosis strains and active transmission of Beijing sublineage in Jayapura, Indonesia Papua

Lidya Chaidir, Sarah Sengstake, Jessica de Beer, Antonius Oktavian, Hana Krismawati, Erfin Muhapril, Inri Kusumadewi, Jessi Annisa, Richard Anthony, Dick van Soolingen, Tri Hanggono Achmad, Sangkot Marzuki, Bachti Alisjahbana, Reinout van Crevel

Research output: Contribution to journalA1: Web of Science-article

Abstract

Mycobacterium tuberculosis genotype distribution is different between West and Central Indonesia, but there are no data on the most Eastern part, Papua. We aimed to identify the predominant genotypes of M. tuberculosis responsible for tuberculosis in coastal Papua, their transmission, and the association with patient characteristics. A total of 199 M. tuberculosis isolates were collected. Spoligotyping was applied to describe the population structure of M. tuberculosis, lineage identification was performed using a combination of lineage-specific markers, and genotypic clusters were identified using a combination of 24-locus-MIRU-VNTR and spoligotyping. A high degree of genetic diversity was observed among isolates based on their spoligopatterns. Strains from modern lineage 4 made up almost half of strains (46.9%), being more abundant than the ancient lineage 1 (33.7%), and modern lineage 2 (19.4%). Thirty-five percent of strains belonged to genotypic clusters, especially strains in the Beijing genotype. Previous TB treatment and mutations associated with drug resistance were more common in patients infected with strains of the Beijing genotype. Papua shows a different distribution of M. tuberculosis genotypes compared to other parts of Indonesia. Clustering and drug resistance of modern strains recently introduced to Papua may contribute to the high tuberculosis burden in this region.

Original languageEnglish
JournalInfection, Genetics and Evolution
Volume39
Pages (from-to)187-193
Number of pages7
ISSN1567-1348
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Adult
  • Evolution, Molecular
  • Female
  • Genetic Variation
  • Genotype
  • Humans
  • Indonesia/epidemiology
  • Male
  • Minisatellite Repeats
  • Molecular Typing
  • Mycobacterium tuberculosis/classification
  • Phylogeny
  • Tuberculosis/epidemiology
  • Young Adult

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