Proline-specific peptidase activities (DPP4, PRCP, FAP and PREP) in plasma of hospitalized COVID-19 patients

An Bracke; Emilie De Hert; Michelle De Bruyn; Karen Claesen; Gwendolyn Vliegen; Alexandra Vujkovic; Lida van Petersen; Fien H R De Winter; An Hotterbeekx; Isabel Brosius; Caroline Theunissen; Sabrina Van Ierssel; Maartje van Frankenhuijsen; Erika Vlieghe; Koen Vercauteren; Pieter Van der Veken; Dirk Hendriks; Samir Kumar-Singh; Ingrid De Meester

doi:10.1016/j.cca.2022.03.005

Proline-specific peptidase activities (DPP4, PRCP, FAP and PREP) in plasma of hospitalized COVID-19 patients

An Bracke, Emilie De Hert, Michelle De Bruyn, Karen Claesen, Gwendolyn Vliegen, Alexandra Vujkovic, Lida van Petersen, Fien H R De Winter, An Hotterbeekx, Isabel Brosius, Caroline Theunissen, Sabrina Van Ierssel, Maartje van Frankenhuijsen, Erika Vlieghe, Koen Vercauteren, Pieter Van der Veken, Dirk Hendriks, Samir Kumar-Singh, Ingrid De Meester

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

BACKGROUND: COVID-19 patients experience several features of dysregulated immune system observed in sepsis. We previously showed a dysregulation of several proline-selective peptidases such as dipeptidyl peptidase 4 (DPP4), fibroblast activation protein alpha (FAP), prolyl oligopeptidase (PREP) and prolylcarboxypeptidase (PRCP) in sepsis. In this study, we investigated whether these peptidases are similarly dysregulated in hospitalized COVID-19 patients.

METHODS: Fifty-six hospitalized COVID-19 patients and 32 healthy controls were included. Enzymatic activities of DPP4, FAP, PREP and PRCP were measured in samples collected shortly after hospital admission and in longitudinal follow-up samples.

RESULTS: Compared to healthy controls, both DPP4 and FAP activities were significantly lower in COVID-19 patients at hospital admission and FAP activity further decreased significantly in the first week of hospitalization. While PRCP activity remained unchanged, PREP activity was significantly increased in COVID-19 patients at hospitalization and further increased during hospital stay and stayed elevated until the day of discharge.

CONCLUSION: The changes in activities of proline-selective peptidases in plasma are very similar in COVID-19 and septic shock patients. The pronounced decrease in FAP activity deserves further investigation, both from a pathophysiological viewpoint and as its utility as a part of a biomarker panel.

Original language	English
Journal	Clinica Chimica Acta
Volume	531
Pages (from-to)	4-11
Number of pages	8
ISSN	0009-8981
DOIs	https://doi.org/10.1016/j.cca.2022.03.005
Publication status	Published - 2022

Keywords

COVID-19
Carboxypeptidases
Dipeptidyl Peptidase 4
Endopeptidases
Gelatinases
Humans
Membrane Proteins
Peptide Hydrolases
Proline
Serine Endopeptidases
Shock, Septic

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10.1016/j.cca.2022.03.005

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Bracke, A., De Hert, E., De Bruyn, M., Claesen, K., Vliegen, G., Vujkovic, A., van Petersen, L., De Winter, F. H. R., Hotterbeekx, A., Brosius, I., Theunissen, C., Van Ierssel, S., van Frankenhuijsen, M., Vlieghe, E., Vercauteren, K., Van der Veken, P., Hendriks, D., Kumar-Singh, S., & De Meester, I. (2022). Proline-specific peptidase activities (DPP4, PRCP, FAP and PREP) in plasma of hospitalized COVID-19 patients. Clinica Chimica Acta, 531, 4-11. https://doi.org/10.1016/j.cca.2022.03.005

@article{155725cd142b4dbeac083a315b952770,

title = "Proline-specific peptidase activities (DPP4, PRCP, FAP and PREP) in plasma of hospitalized COVID-19 patients",

abstract = "BACKGROUND: COVID-19 patients experience several features of dysregulated immune system observed in sepsis. We previously showed a dysregulation of several proline-selective peptidases such as dipeptidyl peptidase 4 (DPP4), fibroblast activation protein alpha (FAP), prolyl oligopeptidase (PREP) and prolylcarboxypeptidase (PRCP) in sepsis. In this study, we investigated whether these peptidases are similarly dysregulated in hospitalized COVID-19 patients.METHODS: Fifty-six hospitalized COVID-19 patients and 32 healthy controls were included. Enzymatic activities of DPP4, FAP, PREP and PRCP were measured in samples collected shortly after hospital admission and in longitudinal follow-up samples.RESULTS: Compared to healthy controls, both DPP4 and FAP activities were significantly lower in COVID-19 patients at hospital admission and FAP activity further decreased significantly in the first week of hospitalization. While PRCP activity remained unchanged, PREP activity was significantly increased in COVID-19 patients at hospitalization and further increased during hospital stay and stayed elevated until the day of discharge.CONCLUSION: The changes in activities of proline-selective peptidases in plasma are very similar in COVID-19 and septic shock patients. The pronounced decrease in FAP activity deserves further investigation, both from a pathophysiological viewpoint and as its utility as a part of a biomarker panel.",

keywords = "COVID-19, Carboxypeptidases, Dipeptidyl Peptidase 4, Endopeptidases, Gelatinases, Humans, Membrane Proteins, Peptide Hydrolases, Proline, Serine Endopeptidases, Shock, Septic",

author = "An Bracke and {De Hert}, Emilie and {De Bruyn}, Michelle and Karen Claesen and Gwendolyn Vliegen and Alexandra Vujkovic and {van Petersen}, Lida and {De Winter}, {Fien H R} and An Hotterbeekx and Isabel Brosius and Caroline Theunissen and {Van Ierssel}, Sabrina and {van Frankenhuijsen}, Maartje and Erika Vlieghe and Koen Vercauteren and {Van der Veken}, Pieter and Dirk Hendriks and Samir Kumar-Singh and {De Meester}, Ingrid",

year = "2022",

doi = "10.1016/j.cca.2022.03.005",

language = "English",

volume = "531",

pages = "4--11",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier",

}

Bracke, A, De Hert, E, De Bruyn, M, Claesen, K, Vliegen, G, Vujkovic, A, van Petersen, L, De Winter, FHR, Hotterbeekx, A, Brosius, I , Theunissen, C, Van Ierssel, S, van Frankenhuijsen, M , Vlieghe, E , Vercauteren, K, Van der Veken, P, Hendriks, D, Kumar-Singh, S & De Meester, I 2022, 'Proline-specific peptidase activities (DPP4, PRCP, FAP and PREP) in plasma of hospitalized COVID-19 patients', Clinica Chimica Acta, vol. 531, pp. 4-11. https://doi.org/10.1016/j.cca.2022.03.005

TY - JOUR

T1 - Proline-specific peptidase activities (DPP4, PRCP, FAP and PREP) in plasma of hospitalized COVID-19 patients

AU - Bracke, An

AU - De Hert, Emilie

AU - De Bruyn, Michelle

AU - Claesen, Karen

AU - Vliegen, Gwendolyn

AU - Vujkovic, Alexandra

AU - van Petersen, Lida

AU - De Winter, Fien H R

AU - Hotterbeekx, An

AU - Brosius, Isabel

AU - Theunissen, Caroline

AU - Van Ierssel, Sabrina

AU - van Frankenhuijsen, Maartje

AU - Vlieghe, Erika

AU - Vercauteren, Koen

AU - Van der Veken, Pieter

AU - Hendriks, Dirk

AU - Kumar-Singh, Samir

AU - De Meester, Ingrid

PY - 2022

Y1 - 2022

N2 - BACKGROUND: COVID-19 patients experience several features of dysregulated immune system observed in sepsis. We previously showed a dysregulation of several proline-selective peptidases such as dipeptidyl peptidase 4 (DPP4), fibroblast activation protein alpha (FAP), prolyl oligopeptidase (PREP) and prolylcarboxypeptidase (PRCP) in sepsis. In this study, we investigated whether these peptidases are similarly dysregulated in hospitalized COVID-19 patients.METHODS: Fifty-six hospitalized COVID-19 patients and 32 healthy controls were included. Enzymatic activities of DPP4, FAP, PREP and PRCP were measured in samples collected shortly after hospital admission and in longitudinal follow-up samples.RESULTS: Compared to healthy controls, both DPP4 and FAP activities were significantly lower in COVID-19 patients at hospital admission and FAP activity further decreased significantly in the first week of hospitalization. While PRCP activity remained unchanged, PREP activity was significantly increased in COVID-19 patients at hospitalization and further increased during hospital stay and stayed elevated until the day of discharge.CONCLUSION: The changes in activities of proline-selective peptidases in plasma are very similar in COVID-19 and septic shock patients. The pronounced decrease in FAP activity deserves further investigation, both from a pathophysiological viewpoint and as its utility as a part of a biomarker panel.

AB - BACKGROUND: COVID-19 patients experience several features of dysregulated immune system observed in sepsis. We previously showed a dysregulation of several proline-selective peptidases such as dipeptidyl peptidase 4 (DPP4), fibroblast activation protein alpha (FAP), prolyl oligopeptidase (PREP) and prolylcarboxypeptidase (PRCP) in sepsis. In this study, we investigated whether these peptidases are similarly dysregulated in hospitalized COVID-19 patients.METHODS: Fifty-six hospitalized COVID-19 patients and 32 healthy controls were included. Enzymatic activities of DPP4, FAP, PREP and PRCP were measured in samples collected shortly after hospital admission and in longitudinal follow-up samples.RESULTS: Compared to healthy controls, both DPP4 and FAP activities were significantly lower in COVID-19 patients at hospital admission and FAP activity further decreased significantly in the first week of hospitalization. While PRCP activity remained unchanged, PREP activity was significantly increased in COVID-19 patients at hospitalization and further increased during hospital stay and stayed elevated until the day of discharge.CONCLUSION: The changes in activities of proline-selective peptidases in plasma are very similar in COVID-19 and septic shock patients. The pronounced decrease in FAP activity deserves further investigation, both from a pathophysiological viewpoint and as its utility as a part of a biomarker panel.

KW - COVID-19

KW - Carboxypeptidases

KW - Dipeptidyl Peptidase 4

KW - Endopeptidases

KW - Gelatinases

KW - Humans

KW - Membrane Proteins

KW - Peptide Hydrolases

KW - Proline

KW - Serine Endopeptidases

KW - Shock, Septic

U2 - 10.1016/j.cca.2022.03.005

DO - 10.1016/j.cca.2022.03.005

M3 - A1: Web of Science-article

C2 - 35283094

SN - 0009-8981

VL - 531

SP - 4

EP - 11

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

ER -

Proline-specific peptidase activities (DPP4, PRCP, FAP and PREP) in plasma of hospitalized COVID-19 patients

Abstract

Keywords

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