Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection

Koen O. A. Vercauteren, Annelies Keymeulen, Ludo Mahieu, Veerle Cossey, Alexandra Casaer, Christine Van Mol, Koenraad Smets, Elizaveta Padalko

Research output: Contribution to journalA1: Web of Science-article

Abstract

To evaluate the potential of dried blood spots (DBS) as a congenital cytomegalovirus (cCMV) testing specimen, the laboratory diagnostic accuracy of polymerase chain reaction (PCR) on DBS was compared to viral urine cultures from neonates suspected for cCMV. Two different extraction methods (EasyMAG, bioMerieux versus Qiagen) and 2 real-time PCR protocols (in-house versus Argene) were compared. We were able to collect both DBS and urine samples in 6 Belgian neonatal units from 276 neonates suspected for cCMV registered in CMVREG (an online neonatal registry system). Forty-eight neonates (17.4%) were positive by viral culture in urine. Laboratory diagnostic accuracy parameters of DBS-PCR were both extraction method and PCR protocol dependent. Not all DBS-CMV-PCR methods successfully detected urine-culture-positive neonates born after first-trimester seroconversions. Interestingly, however, all urine-culture-positive neonates having clinical signs of cCMV did consistently score positive. Keywords: Cytomegalovirus Congenital Dried blood spot testing (C) 2020 Elsevier Inc. All rights reserved.

Original languageEnglish
Article number115051
JournalDiagnostic Microbiology and Infectious Disease
Volume97
Issue number3
Number of pages6
ISSN0732-8893
DOIs
Publication statusPublished - 2020
Externally publishedYes

Keywords

  • Cytomegalovirus
  • Congenital
  • Dried blood spot testing
  • CHAIN-REACTION ASSAYS
  • HEARING-LOSS
  • DNA DETECTION
  • NEWBORNS
  • DIAGNOSIS
  • CHILDREN
  • FOLLOW

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