Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection

Koen O. A. Vercauteren; Annelies Keymeulen; Ludo Mahieu; Veerle Cossey; Alexandra Casaer; Christine Van Mol; Koenraad Smets; Elizaveta Padalko

doi:10.1016/j.diagmicrobio.2020.115051

Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection

Koen O. A. Vercauteren, Annelies Keymeulen, Ludo Mahieu, Veerle Cossey, Alexandra Casaer, Christine Van Mol, Koenraad Smets, Elizaveta Padalko

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

To evaluate the potential of dried blood spots (DBS) as a congenital cytomegalovirus (cCMV) testing specimen, the laboratory diagnostic accuracy of polymerase chain reaction (PCR) on DBS was compared to viral urine cultures from neonates suspected for cCMV. Two different extraction methods (EasyMAG, bioMerieux versus Qiagen) and 2 real-time PCR protocols (in-house versus Argene) were compared. We were able to collect both DBS and urine samples in 6 Belgian neonatal units from 276 neonates suspected for cCMV registered in CMVREG (an online neonatal registry system). Forty-eight neonates (17.4%) were positive by viral culture in urine. Laboratory diagnostic accuracy parameters of DBS-PCR were both extraction method and PCR protocol dependent. Not all DBS-CMV-PCR methods successfully detected urine-culture-positive neonates born after first-trimester seroconversions. Interestingly, however, all urine-culture-positive neonates having clinical signs of cCMV did consistently score positive. Keywords: Cytomegalovirus Congenital Dried blood spot testing (C) 2020 Elsevier Inc. All rights reserved.

Original language	English
Article number	115051
Journal	Diagnostic Microbiology and Infectious Disease
Volume	97
Issue number	3
Number of pages	6
ISSN	0732-8893
DOIs	https://doi.org/10.1016/j.diagmicrobio.2020.115051
Publication status	Published - 2020
Externally published	Yes

Keywords

Cytomegalovirus
Congenital
Dried blood spot testing
CHAIN-REACTION ASSAYS
HEARING-LOSS
DNA DETECTION
NEWBORNS
DIAGNOSIS
CHILDREN
FOLLOW

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Vercauteren, K. O. A., Keymeulen, A., Mahieu, L., Cossey, V., Casaer, A., Van Mol, C., Smets, K., & Padalko, E. (2020). Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection. Diagnostic Microbiology and Infectious Disease, 97(3), Article 115051. https://doi.org/10.1016/j.diagmicrobio.2020.115051

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title = "Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection",

abstract = "To evaluate the potential of dried blood spots (DBS) as a congenital cytomegalovirus (cCMV) testing specimen, the laboratory diagnostic accuracy of polymerase chain reaction (PCR) on DBS was compared to viral urine cultures from neonates suspected for cCMV. Two different extraction methods (EasyMAG, bioMerieux versus Qiagen) and 2 real-time PCR protocols (in-house versus Argene) were compared. We were able to collect both DBS and urine samples in 6 Belgian neonatal units from 276 neonates suspected for cCMV registered in CMVREG (an online neonatal registry system). Forty-eight neonates (17.4%) were positive by viral culture in urine. Laboratory diagnostic accuracy parameters of DBS-PCR were both extraction method and PCR protocol dependent. Not all DBS-CMV-PCR methods successfully detected urine-culture-positive neonates born after first-trimester seroconversions. Interestingly, however, all urine-culture-positive neonates having clinical signs of cCMV did consistently score positive. Keywords: Cytomegalovirus Congenital Dried blood spot testing (C) 2020 Elsevier Inc. All rights reserved.",

keywords = "Cytomegalovirus, Congenital, Dried blood spot testing, CHAIN-REACTION ASSAYS, HEARING-LOSS, DNA DETECTION, NEWBORNS, DIAGNOSIS, CHILDREN, FOLLOW",

author = "Vercauteren, {Koen O. A.} and Annelies Keymeulen and Ludo Mahieu and Veerle Cossey and Alexandra Casaer and {Van Mol}, Christine and Koenraad Smets and Elizaveta Padalko",

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Vercauteren, KOA, Keymeulen, A, Mahieu, L, Cossey, V, Casaer, A, Van Mol, C, Smets, K & Padalko, E 2020, 'Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection', Diagnostic Microbiology and Infectious Disease, vol. 97, no. 3, 115051. https://doi.org/10.1016/j.diagmicrobio.2020.115051

Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection. / Vercauteren, Koen O. A.; Keymeulen, Annelies; Mahieu, Ludo et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 97, No. 3, 115051, 2020.

Research output: Contribution to journal › A1: Web of Science-article › peer-review

TY - JOUR

T1 - Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection

AU - Vercauteren, Koen O. A.

AU - Keymeulen, Annelies

AU - Mahieu, Ludo

AU - Cossey, Veerle

AU - Casaer, Alexandra

AU - Van Mol, Christine

AU - Smets, Koenraad

AU - Padalko, Elizaveta

N1 - NPP; PPT

PY - 2020

Y1 - 2020

N2 - To evaluate the potential of dried blood spots (DBS) as a congenital cytomegalovirus (cCMV) testing specimen, the laboratory diagnostic accuracy of polymerase chain reaction (PCR) on DBS was compared to viral urine cultures from neonates suspected for cCMV. Two different extraction methods (EasyMAG, bioMerieux versus Qiagen) and 2 real-time PCR protocols (in-house versus Argene) were compared. We were able to collect both DBS and urine samples in 6 Belgian neonatal units from 276 neonates suspected for cCMV registered in CMVREG (an online neonatal registry system). Forty-eight neonates (17.4%) were positive by viral culture in urine. Laboratory diagnostic accuracy parameters of DBS-PCR were both extraction method and PCR protocol dependent. Not all DBS-CMV-PCR methods successfully detected urine-culture-positive neonates born after first-trimester seroconversions. Interestingly, however, all urine-culture-positive neonates having clinical signs of cCMV did consistently score positive. Keywords: Cytomegalovirus Congenital Dried blood spot testing (C) 2020 Elsevier Inc. All rights reserved.

AB - To evaluate the potential of dried blood spots (DBS) as a congenital cytomegalovirus (cCMV) testing specimen, the laboratory diagnostic accuracy of polymerase chain reaction (PCR) on DBS was compared to viral urine cultures from neonates suspected for cCMV. Two different extraction methods (EasyMAG, bioMerieux versus Qiagen) and 2 real-time PCR protocols (in-house versus Argene) were compared. We were able to collect both DBS and urine samples in 6 Belgian neonatal units from 276 neonates suspected for cCMV registered in CMVREG (an online neonatal registry system). Forty-eight neonates (17.4%) were positive by viral culture in urine. Laboratory diagnostic accuracy parameters of DBS-PCR were both extraction method and PCR protocol dependent. Not all DBS-CMV-PCR methods successfully detected urine-culture-positive neonates born after first-trimester seroconversions. Interestingly, however, all urine-culture-positive neonates having clinical signs of cCMV did consistently score positive. Keywords: Cytomegalovirus Congenital Dried blood spot testing (C) 2020 Elsevier Inc. All rights reserved.

KW - Cytomegalovirus

KW - Congenital

KW - Dried blood spot testing

KW - CHAIN-REACTION ASSAYS

KW - HEARING-LOSS

KW - DNA DETECTION

KW - NEWBORNS

KW - DIAGNOSIS

KW - CHILDREN

KW - FOLLOW

U2 - 10.1016/j.diagmicrobio.2020.115051

DO - 10.1016/j.diagmicrobio.2020.115051

M3 - A1: Web of Science-article

SN - 0732-8893

VL - 97

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

M1 - 115051

ER -

Vercauteren KOA, Keymeulen A, Mahieu L, Cossey V, Casaer A, Van Mol C et al. Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection. Diagnostic Microbiology and Infectious Disease. 2020;97(3):115051. doi: 10.1016/j.diagmicrobio.2020.115051

Prospective multicenter comparison of urine culture with PCR on dried blood spots using 2 different extraction and PCR methods in neonates suspected for congenital cytomegalovirus infection

Abstract

Keywords

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