TY - JOUR
T1 - Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster
AU - Brustolin, Marco
AU - Rodon, Jordi
AU - Rodríguez de la Concepción, María Luisa
AU - Ávila-Nieto, Carlos
AU - Cantero, Guillermo
AU - Pérez, Mónica
AU - Te, Nigeer
AU - Noguera-Julián, Marc
AU - Guallar, Víctor
AU - Valencia, Alfonso
AU - Roca, Núria
AU - Izquierdo-Useros, Nuria
AU - Blanco, Julià
AU - Clotet, Bonaventura
AU - Bensaid, Albert
AU - Carrillo, Jorge
AU - Vergara-Alert, Júlia
AU - Segalés, Joaquim
N1 - FTX; DOAJ; (CC BY-NC 4.0)
PY - 2021
Y1 - 2021
N2 - Reinfections with SARS-CoV-2 have already been documented in humans, although its real incidence is currently unknown. Besides having a great impact on public health, this phenomenon raises the question of immunity generated by a single infection is sufficient to provide sterilizing/protective immunity to a subsequent SARS-CoV-2 re-exposure. The Golden Syrian hamster is a manageable animal model to explore immunological mechanisms able to counteract COVID-19, as it recapitulates pathological aspects of mild to moderately affected patients. Here, we report that SARS-CoV-2-inoculated hamsters resolve infection in the upper and lower respiratory tracts within seven days upon inoculation with the Cat01 (G614) SARS-CoV-2 isolate. Three weeks after the primary challenge, and despite high titres of neutralizing antibodies, half of the animals were susceptible to reinfection by both identical (Cat01, G614) and variant (WA/1, D614) SARS-CoV-2 isolates. However, upon re-inoculation, only nasal tissues were transiently infected with much lower viral replication than those observed after the first inoculation. These data indicate that a primary SARS-CoV-2 infection is not sufficient to elicit a sterilizing immunity in hamster models but protects against lung disease.
AB - Reinfections with SARS-CoV-2 have already been documented in humans, although its real incidence is currently unknown. Besides having a great impact on public health, this phenomenon raises the question of immunity generated by a single infection is sufficient to provide sterilizing/protective immunity to a subsequent SARS-CoV-2 re-exposure. The Golden Syrian hamster is a manageable animal model to explore immunological mechanisms able to counteract COVID-19, as it recapitulates pathological aspects of mild to moderately affected patients. Here, we report that SARS-CoV-2-inoculated hamsters resolve infection in the upper and lower respiratory tracts within seven days upon inoculation with the Cat01 (G614) SARS-CoV-2 isolate. Three weeks after the primary challenge, and despite high titres of neutralizing antibodies, half of the animals were susceptible to reinfection by both identical (Cat01, G614) and variant (WA/1, D614) SARS-CoV-2 isolates. However, upon re-inoculation, only nasal tissues were transiently infected with much lower viral replication than those observed after the first inoculation. These data indicate that a primary SARS-CoV-2 infection is not sufficient to elicit a sterilizing immunity in hamster models but protects against lung disease.
KW - Animals
KW - Antibodies, Neutralizing/immunology
KW - Antibodies, Viral/immunology
KW - COVID-19/diagnosis
KW - Cell Line
KW - Cricetinae
KW - Disease Models, Animal
KW - Female
KW - Host-Pathogen Interactions/immunology
KW - Humans
KW - Immunity, Humoral
KW - Immunohistochemistry
KW - Male
KW - Neutralization Tests
KW - Reinfection/virology
KW - SARS-CoV-2/genetics
KW - Viral Load
KW - Virus Replication
U2 - 10.1080/22221751.2021.1913974
DO - 10.1080/22221751.2021.1913974
M3 - A1: Web of Science-article
C2 - 33825619
SN - 2222-1751
VL - 10
SP - 797
EP - 809
JO - Emerging Microbes & Infections
JF - Emerging Microbes & Infections
IS - 1
ER -