Proteomic analysis of Taenia solium metacestode excretion-secretion proteins

B. Victor, K. Kanobana, S. Gabriël, K. Polman, N. Deckers, P. Dorny, A.M. Deelder, M. Palmblad

Research output: Contribution to journalA1: Web of Science-article

Abstract

The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better understanding of the interaction between T. solium metacestodes and their host, there is a need for more information about the proteins that are released by the parasite. In this study, we used protein sequences from different helminths, 1-D gel electrophoresis, reversed-phase liquid chromatography, and tandem mass spectrometry to analyze the excretion-secretion proteins produced by T. solium metacestodes from infected pigs. This is the first report of the T. solium metacestode excretion-secretion proteome. We report 76 proteins including 27 already described T. solium proteins, 17 host proteins and 32 proteins likely to be of T. solium origin, but identified using sequences from other helminths.
Original languageEnglish
JournalProteomics
Volume12
Issue number11
Pages (from-to)1860-1869
Number of pages10
ISSN1615-9853
DOIs
Publication statusPublished - 2012

Keywords

  • Helminthic diseases
  • Cysticercosis
  • Taenia solium
  • Echinococcus multilocularis
  • Pigs
  • Metacestodes
  • Proteomics
  • Mass spectrometry
  • Proteins
  • Sequencing
  • Zambia
  • Africa-Southern
  • Peru
  • America-Latin

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