Rapid diagnostic tests for malaria diagnosis in the Peruvian Amazon: impact of pfhrp2 gene deletions and cross-reactions

J. Maltha, D. Gamboa, J. Bendezu, L. Sanchez, L. Cnops, P. Gillet, J. Jacobs

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Abstract

BACKGROUND: In the Peruvian Amazon, Plasmodium falciparum and Plasmodium vivax malaria are endemic in rural areas, where microscopy is not available. Malaria rapid diagnostic tests (RDTs) provide quick and accurate diagnosis. However, pfhrp2 gene deletions may limit the use of histidine-rich protein-2 (PfHRP2) detecting RDTs. Further, cross-reactions of P. falciparum with P. vivax-specific test lines and vice versa may impair diagnostic specificity. METHODS: Thirteen RDT products were evaluated on 179 prospectively collected malaria positive samples. Species diagnosis was performed by microscopy and confirmed by PCR. Pfhrp2 gene deletions were assessed by PCR. RESULTS: Sensitivity for P. falciparum diagnosis was lower for PfHRP2 compared to P. falciparum-specific Plasmodium lactate dehydrogenase (Pf-pLDH)-detecting RDTs (71.6% vs. 98.7%, p
Original languageEnglish
Article numbere43094
JournalPLoS ONE
Volume7
Issue number8
ISSN1932-6203
DOIs
Publication statusPublished - 2012

Keywords

  • Protozoal diseases
  • Malaria
  • Plasmodium falciparum
  • Plasmodium vivax
  • Vectors
  • Mosquitoes
  • Anopheles
  • Evaluation
  • Rapid diagnostic tests
  • Performance
  • Specificity
  • Sensitivity
  • Lactate dehydrogenase
  • False-negative
  • Cross reactions
  • Amazona
  • Forest
  • Laboratory techniques and procedures
  • Peru
  • America-Latin

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