Rapid diagnostic tests for malaria diagnosis in the Peruvian Amazon: impact of pfhrp2 gene deletions and cross-reactions

J. Maltha; D. Gamboa; J. Bendezu; L. Sanchez; L. Cnops; P. Gillet; J. Jacobs

doi:10.1371/journal.pone.0043094

Rapid diagnostic tests for malaria diagnosis in the Peruvian Amazon: impact of pfhrp2 gene deletions and cross-reactions

J. Maltha, D. Gamboa, J. Bendezu, L. Sanchez, L. Cnops, P. Gillet, J. Jacobs

Tropical Bacteriology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

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Abstract

BACKGROUND: In the Peruvian Amazon, Plasmodium falciparum and Plasmodium vivax malaria are endemic in rural areas, where microscopy is not available. Malaria rapid diagnostic tests (RDTs) provide quick and accurate diagnosis. However, pfhrp2 gene deletions may limit the use of histidine-rich protein-2 (PfHRP2) detecting RDTs. Further, cross-reactions of P. falciparum with P. vivax-specific test lines and vice versa may impair diagnostic specificity. METHODS: Thirteen RDT products were evaluated on 179 prospectively collected malaria positive samples. Species diagnosis was performed by microscopy and confirmed by PCR. Pfhrp2 gene deletions were assessed by PCR. RESULTS: Sensitivity for P. falciparum diagnosis was lower for PfHRP2 compared to P. falciparum-specific Plasmodium lactate dehydrogenase (Pf-pLDH)-detecting RDTs (71.6% vs. 98.7%, p

Original language	English
Article number	e43094
Journal	PLoS ONE
Volume	7
Issue number	8
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0043094
Publication status	Published - 2012

Keywords

Protozoal diseases
Malaria
Plasmodium falciparum
Plasmodium vivax
Vectors
Mosquitoes
Anopheles
Evaluation
Rapid diagnostic tests
Performance
Specificity
Sensitivity
Lactate dehydrogenase
False-negative
Cross reactions
Amazona
Forest
Laboratory techniques and procedures
Peru
America-Latin

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title = "Rapid diagnostic tests for malaria diagnosis in the Peruvian Amazon: impact of pfhrp2 gene deletions and cross-reactions",

abstract = "BACKGROUND: In the Peruvian Amazon, Plasmodium falciparum and Plasmodium vivax malaria are endemic in rural areas, where microscopy is not available. Malaria rapid diagnostic tests (RDTs) provide quick and accurate diagnosis. However, pfhrp2 gene deletions may limit the use of histidine-rich protein-2 (PfHRP2) detecting RDTs. Further, cross-reactions of P. falciparum with P. vivax-specific test lines and vice versa may impair diagnostic specificity. METHODS: Thirteen RDT products were evaluated on 179 prospectively collected malaria positive samples. Species diagnosis was performed by microscopy and confirmed by PCR. Pfhrp2 gene deletions were assessed by PCR. RESULTS: Sensitivity for P. falciparum diagnosis was lower for PfHRP2 compared to P. falciparum-specific Plasmodium lactate dehydrogenase (Pf-pLDH)-detecting RDTs (71.6% vs. 98.7%, p",

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author = "J. Maltha and D. Gamboa and J. Bendezu and L. Sanchez and L. Cnops and P. Gillet and J. Jacobs",

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T1 - Rapid diagnostic tests for malaria diagnosis in the Peruvian Amazon: impact of pfhrp2 gene deletions and cross-reactions

AU - Maltha, J.

AU - Gamboa, D.

AU - Bendezu, J.

AU - Sanchez, L.

AU - Cnops, L.

AU - Gillet, P.

AU - Jacobs, J.

N1 - ITG-C1B; ITG-C5B; ITG-C6A; ITG-CLA; DCS; U-TLM; JIF; DOI; PDF; E-only; Abstract; DSPACE

PY - 2012

Y1 - 2012

N2 - BACKGROUND: In the Peruvian Amazon, Plasmodium falciparum and Plasmodium vivax malaria are endemic in rural areas, where microscopy is not available. Malaria rapid diagnostic tests (RDTs) provide quick and accurate diagnosis. However, pfhrp2 gene deletions may limit the use of histidine-rich protein-2 (PfHRP2) detecting RDTs. Further, cross-reactions of P. falciparum with P. vivax-specific test lines and vice versa may impair diagnostic specificity. METHODS: Thirteen RDT products were evaluated on 179 prospectively collected malaria positive samples. Species diagnosis was performed by microscopy and confirmed by PCR. Pfhrp2 gene deletions were assessed by PCR. RESULTS: Sensitivity for P. falciparum diagnosis was lower for PfHRP2 compared to P. falciparum-specific Plasmodium lactate dehydrogenase (Pf-pLDH)-detecting RDTs (71.6% vs. 98.7%, p

AB - BACKGROUND: In the Peruvian Amazon, Plasmodium falciparum and Plasmodium vivax malaria are endemic in rural areas, where microscopy is not available. Malaria rapid diagnostic tests (RDTs) provide quick and accurate diagnosis. However, pfhrp2 gene deletions may limit the use of histidine-rich protein-2 (PfHRP2) detecting RDTs. Further, cross-reactions of P. falciparum with P. vivax-specific test lines and vice versa may impair diagnostic specificity. METHODS: Thirteen RDT products were evaluated on 179 prospectively collected malaria positive samples. Species diagnosis was performed by microscopy and confirmed by PCR. Pfhrp2 gene deletions were assessed by PCR. RESULTS: Sensitivity for P. falciparum diagnosis was lower for PfHRP2 compared to P. falciparum-specific Plasmodium lactate dehydrogenase (Pf-pLDH)-detecting RDTs (71.6% vs. 98.7%, p

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KW - Malaria

KW - Plasmodium falciparum

KW - Plasmodium vivax

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KW - Evaluation

KW - Rapid diagnostic tests

KW - Performance

KW - Specificity

KW - Sensitivity

KW - Lactate dehydrogenase

KW - False-negative

KW - Cross reactions

KW - Amazona

KW - Forest

KW - Laboratory techniques and procedures

KW - Peru

KW - America-Latin

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DO - 10.1371/journal.pone.0043094

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JO - PLoS ONE

JF - PLoS ONE

IS - 8

M1 - e43094

ER -

Rapid diagnostic tests for malaria diagnosis in the Peruvian Amazon: impact of pfhrp2 gene deletions and cross-reactions

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Keywords

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