Rapid low-cost identification of Mycobacterium tuberculosis complex using p-nitro-benzoic acid (PNB) as inhibitor and the resazurin microplate assay (REMA): a preliminary study

D. Affolabi; N. Sanoussi; M. Odoun; A. Martin; L. Koukpemedji; J.C. Palomino; L. Kestens; S. Anagonou; F. Portaels

doi:10.5897/AJMR10.278

Rapid low-cost identification of Mycobacterium tuberculosis complex using p-nitro-benzoic acid (PNB) as inhibitor and the resazurin microplate assay (REMA): a preliminary study

D. Affolabi
, N. Sanoussi
, M. Odoun
, A. Martin
, L. Koukpemedji
, J.C. Palomino
, L. Kestens
, S. Anagonou
, F. Portaels

Mycobacteriology

Research output: Contribution to journal › A1: Peer-reviewed journal articles › peer-review

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Abstract

A rapid test for the differentiation of Mycobacterium tuberculosis complex from non-tuberculous mycobacteria (NTM) was developed using p-nitro-benzoic acid (PNB) as inhibitor and the resazurin microplate assay (REMA) for detection of growth. One hundred and fifty-one M. tuberculosis strains and 36 NTM were evaluated. Using a cut-off of 250 µg/ml of PNB, all M. tuberculosis strains were correctly differentiated from NTM; only two NTM strains failed to be correctly identified with this procedure. The time to obtain results was 8 days compared to 28 days or more with the conventional method. The use of REMA and PNB represents a rapid and inexpensive procedure that could be used in laboratories in low-income settings for the rapid differentiation between M. tuberculosiscomplex and NTM.

Original language	English
Journal	African Journal of Microbiology Research
Volume	7
Issue number	24
Pages (from-to)	3135-3138
Number of pages	4
ISSN	1996-0808
DOIs	https://doi.org/10.5897/AJMR10.278
Publication status	Published - 2013

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2013ajmr3135Final published version, 268 KBLicence: CC BY

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Affolabi, D., Sanoussi, N., Odoun, M., Martin, A., Koukpemedji, L., Palomino , J. C., Kestens, L., Anagonou, S., & Portaels, F. (2013). Rapid low-cost identification of Mycobacterium tuberculosis complex using p-nitro-benzoic acid (PNB) as inhibitor and the resazurin microplate assay (REMA): a preliminary study. African Journal of Microbiology Research, 7(24), 3135-3138. https://doi.org/10.5897/AJMR10.278

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abstract = "A rapid test for the differentiation of Mycobacterium tuberculosis complex from non-tuberculous mycobacteria (NTM) was developed using p-nitro-benzoic acid (PNB) as inhibitor and the resazurin microplate assay (REMA) for detection of growth. One hundred and fifty-one M. tuberculosis strains and 36 NTM were evaluated. Using a cut-off of 250 µg/ml of PNB, all M. tuberculosis strains were correctly differentiated from NTM; only two NTM strains failed to be correctly identified with this procedure. The time to obtain results was 8 days compared to 28 days or more with the conventional method. The use of REMA and PNB represents a rapid and inexpensive procedure that could be used in laboratories in low-income settings for the rapid differentiation between M. tuberculosiscomplex and NTM.",

keywords = "Bacterial diseases, Tuberculosis, Mycobacterium tuberculosis complex, Identification, Rapid diagnostic tests, Inhibitory activity, Resazurin, Microplate assay, Strains, Concentration, Laboratory techniques and procedures",

author = "D. Affolabi and N. Sanoussi and M. Odoun and A. Martin and L. Koukpemedji and J.C. Palomino and L. Kestens and S. Anagonou and F. Portaels",

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Affolabi, D, Sanoussi, N, Odoun, M, Martin, A, Koukpemedji, L, Palomino , JC, Kestens, L, Anagonou, S & Portaels, F 2013, 'Rapid low-cost identification of Mycobacterium tuberculosis complex using p-nitro-benzoic acid (PNB) as inhibitor and the resazurin microplate assay (REMA): a preliminary study', African Journal of Microbiology Research, vol. 7, no. 24, pp. 3135-3138. https://doi.org/10.5897/AJMR10.278

Rapid low-cost identification of Mycobacterium tuberculosis complex using p-nitro-benzoic acid (PNB) as inhibitor and the resazurin microplate assay (REMA): a preliminary study. / Affolabi, D.; Sanoussi, N.; Odoun, M. et al.
In: African Journal of Microbiology Research, Vol. 7, No. 24, 2013, p. 3135-3138.

Research output: Contribution to journal › A1: Peer-reviewed journal articles › peer-review

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AU - Affolabi, D.

AU - Sanoussi, N.

AU - Odoun, M.

AU - Martin, A.

AU - Koukpemedji, L.

AU - Palomino , J.C.

AU - Kestens, L.

AU - Anagonou, S.

AU - Portaels, F.

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AB - A rapid test for the differentiation of Mycobacterium tuberculosis complex from non-tuberculous mycobacteria (NTM) was developed using p-nitro-benzoic acid (PNB) as inhibitor and the resazurin microplate assay (REMA) for detection of growth. One hundred and fifty-one M. tuberculosis strains and 36 NTM were evaluated. Using a cut-off of 250 µg/ml of PNB, all M. tuberculosis strains were correctly differentiated from NTM; only two NTM strains failed to be correctly identified with this procedure. The time to obtain results was 8 days compared to 28 days or more with the conventional method. The use of REMA and PNB represents a rapid and inexpensive procedure that could be used in laboratories in low-income settings for the rapid differentiation between M. tuberculosiscomplex and NTM.

KW - Bacterial diseases

KW - Tuberculosis

KW - Mycobacterium tuberculosis complex

KW - Identification

KW - Rapid diagnostic tests

KW - Inhibitory activity

KW - Resazurin

KW - Microplate assay

KW - Strains

KW - Concentration

KW - Laboratory techniques and procedures

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DO - 10.5897/AJMR10.278

M3 - A1: Peer-reviewed journal articles

SN - 1996-0808

VL - 7

SP - 3135

EP - 3138

JO - African Journal of Microbiology Research

JF - African Journal of Microbiology Research

IS - 24

ER -

Rapid low-cost identification of Mycobacterium tuberculosis complex using p-nitro-benzoic acid (PNB) as inhibitor and the resazurin microplate assay (REMA): a preliminary study

Abstract

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