Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis

M Mestdagh; PA Fonteyne; L Realini; R Rossau; G Jannes; W Mijs; KAL De Smet; F Portaels; E Van den Eeckhout

doi:10.1128/AAC.43.9.2317

Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis

M Mestdagh, PA Fonteyne, L Realini, R Rossau, G Jannes, W Mijs, KAL De Smet, F Portaels, E Van den Eeckhout

Mycobacteriology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Sixty-two Mycobacterium tuberculosis isolates were tested for pyrazinamidase activity, and their pyrazinamide susceptibility was determined by the radiometric method. Sequencing ofpncA genes in the 23 resistant strains revealed mutations in 16 pyrazinamidase-negative strains, 11 of which had not been previously described. Six isolates containing wild-typepncA might possess alternative resistance mechanisms.

Original language	English
Journal	Antimicrobial Agents and Chemotherapy
Volume	43
Issue number	9
Pages (from-to)	2317-2319
Number of pages	3
ISSN	0066-4804
DOIs	https://doi.org/10.1128/AAC.43.9.2317
Publication status	Published - 1999

Keywords

B780-tropical-medicine
Bacterial diseases
Mycobacterium tuberculosis
Drug resistance
Pyrazinamide
Experimental

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10.1128/AAC.43.9.2317

http://lib.itg.be/pdf/itg/1999/1999aach2317.pdf

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Mestdagh, M., Fonteyne, PA., Realini, L., Rossau, R., Jannes, G., Mijs, W., De Smet, KAL., Portaels, F., & Van den Eeckhout, E. (1999). Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy, 43(9), 2317-2319. https://doi.org/10.1128/AAC.43.9.2317

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title = "Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis",

abstract = "Sixty-two Mycobacterium tuberculosis isolates were tested for pyrazinamidase activity, and their pyrazinamide susceptibility was determined by the radiometric method. Sequencing ofpncA genes in the 23 resistant strains revealed mutations in 16 pyrazinamidase-negative strains, 11 of which had not been previously described. Six isolates containing wild-typepncA might possess alternative resistance mechanisms.",

keywords = "B780-tropical-medicine, Bacterial diseases, Mycobacterium tuberculosis, Drug resistance, Pyrazinamide, Experimental",

author = "M Mestdagh and PA Fonteyne and L Realini and R Rossau and G Jannes and W Mijs and {De Smet}, KAL and F Portaels and {Van den Eeckhout}, E",

note = "FTX: Abonnement",

year = "1999",

doi = "10.1128/AAC.43.9.2317",

language = "English",

volume = "43",

pages = "2317--2319",

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Mestdagh, M, Fonteyne, PA, Realini, L, Rossau, R, Jannes, G, Mijs, W, De Smet, KAL, Portaels, F & Van den Eeckhout, E 1999, 'Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis', Antimicrobial Agents and Chemotherapy, vol. 43, no. 9, pp. 2317-2319. https://doi.org/10.1128/AAC.43.9.2317

Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis. / Mestdagh, M; Fonteyne, PA; Realini, L et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 43, No. 9, 1999, p. 2317-2319.

Research output: Contribution to journal › A1: Web of Science-article › peer-review

TY - JOUR

T1 - Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis

AU - Mestdagh, M

AU - Fonteyne, PA

AU - Realini, L

AU - Rossau, R

AU - Jannes, G

AU - Mijs, W

AU - De Smet, KAL

AU - Portaels, F

AU - Van den Eeckhout, E

N1 - FTX: Abonnement

PY - 1999

Y1 - 1999

N2 - Sixty-two Mycobacterium tuberculosis isolates were tested for pyrazinamidase activity, and their pyrazinamide susceptibility was determined by the radiometric method. Sequencing ofpncA genes in the 23 resistant strains revealed mutations in 16 pyrazinamidase-negative strains, 11 of which had not been previously described. Six isolates containing wild-typepncA might possess alternative resistance mechanisms.

AB - Sixty-two Mycobacterium tuberculosis isolates were tested for pyrazinamidase activity, and their pyrazinamide susceptibility was determined by the radiometric method. Sequencing ofpncA genes in the 23 resistant strains revealed mutations in 16 pyrazinamidase-negative strains, 11 of which had not been previously described. Six isolates containing wild-typepncA might possess alternative resistance mechanisms.

KW - B780-tropical-medicine

KW - Bacterial diseases

KW - Mycobacterium tuberculosis

KW - Drug resistance

KW - Pyrazinamide

KW - Experimental

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000082405700042

U2 - 10.1128/AAC.43.9.2317

DO - 10.1128/AAC.43.9.2317

M3 - A1: Web of Science-article

SN - 0066-4804

VL - 43

SP - 2317

EP - 2319

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 9

ER -

Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis

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Keywords

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