TY - JOUR
T1 - Retrospective cohort analysis for identification of discordant Rifampicin-resistant Xpert MTB/RIF assay results in South Kivu, Eastern Democratic Republic of the Congo, a high burden Tuberculosis setting
AU - Bisimwa, Bertin C
AU - Kiselinova, Maja
AU - Cuella, Isabel
AU - Rigouts, Leen
AU - Bulabula, Andy N H
AU - Byela, Valéry
AU - Chirambiza, Jean-Paul
AU - Mulume, Eric
AU - Birembano, Freddy
AU - Katoto, Patrick P D M
AU - Ngabonziza, Jean-Claude S
AU - Kaswa, Michel
AU - de Jong, Bouke C
AU - Callens, Steven
AU - Bahizire, Esto
N1 - FTX abonnement - AAM ontbreekt
PY - 2025
Y1 - 2025
N2 - BACKGROUND: The Xpert assay has revolutionized the rapid detection of resistance to rifampicin. However, Xpert has its pitfalls. We explored potential determinants of false-positive rifampicin resistance when using Xpert, aiming to refine the precision of TB diagnostics and subsequently contribute to better patient outcomes.METHODS: This is a retrospective cross-sectional analysis of archived Xpert files from the South Kivu province, used to diagnose MTB between 2013 and 2018. Xpert cycle threshold was extracted for each molecular beacon probe and ΔCt was calculated. We used the MTBDRplus line probe assay, which covers the same 81bp RRDR, as reference test.RESULTS: Of 1900 samples positive for MTB, 220 (11.2%) were rifampicin resistant. Of the 141 patients' sputum samples that had results for both Xpert and MTBDRplus, 45 (31.9%) showed discordant results with Xpert, indicating rifampicin resistance while MTBDRplus indicated rifampicin susceptibility, suggesting false-positive rifampicin resistance detection by Xpert, predominantly in samples with very low (Ct>28, OR 2.23, 95% CI 1.30-3.82) or low (Ct 22-28, OR 1.81, 95% CI 1.21-2.71) bacterial loads. Probe E was the most frequently missed probe, followed by multiple probe dropouts or absence of probe binding (OR 1.5, 95% CI 0.731-3.076).CONCLUSION: Our findings indicate that low and very low MTB bacterial loads in sputum is strongly associated with discordant rifampicin resistance results when using Xpert. Further research into underlying mechanisms is needed to establish causality definitively.
AB - BACKGROUND: The Xpert assay has revolutionized the rapid detection of resistance to rifampicin. However, Xpert has its pitfalls. We explored potential determinants of false-positive rifampicin resistance when using Xpert, aiming to refine the precision of TB diagnostics and subsequently contribute to better patient outcomes.METHODS: This is a retrospective cross-sectional analysis of archived Xpert files from the South Kivu province, used to diagnose MTB between 2013 and 2018. Xpert cycle threshold was extracted for each molecular beacon probe and ΔCt was calculated. We used the MTBDRplus line probe assay, which covers the same 81bp RRDR, as reference test.RESULTS: Of 1900 samples positive for MTB, 220 (11.2%) were rifampicin resistant. Of the 141 patients' sputum samples that had results for both Xpert and MTBDRplus, 45 (31.9%) showed discordant results with Xpert, indicating rifampicin resistance while MTBDRplus indicated rifampicin susceptibility, suggesting false-positive rifampicin resistance detection by Xpert, predominantly in samples with very low (Ct>28, OR 2.23, 95% CI 1.30-3.82) or low (Ct 22-28, OR 1.81, 95% CI 1.21-2.71) bacterial loads. Probe E was the most frequently missed probe, followed by multiple probe dropouts or absence of probe binding (OR 1.5, 95% CI 0.731-3.076).CONCLUSION: Our findings indicate that low and very low MTB bacterial loads in sputum is strongly associated with discordant rifampicin resistance results when using Xpert. Further research into underlying mechanisms is needed to establish causality definitively.
UR - https://www.webofscience.com/wos/woscc/full-record/WOS:001575886600001
U2 - 10.1093/cid/ciaf451
DO - 10.1093/cid/ciaf451
M3 - A1: Web of Science-article
C2 - 40796199
SN - 1058-4838
JO - Clinical Infectious Diseases
JF - Clinical Infectious Diseases
M1 - ciaf451
ER -