SARS-CoV-2 mRNA vaccination is not associated with the induction of anti-HLA or non-HLA antibodies

Veerle P W M Wijtvliet, Sonja Verheyden, Barbara Depreter, Christine Heylen, Elke Coeman, Steven Abrams, Benedicte Y De Winter, Annick Massart, Rachel Hellemans, Lissa Pipeleers, Frans H J Claas, Kevin K Ariën, Karl Martin Wissing, Daniel Abramowicz, Kristien J Ledeganck

Research output: Contribution to journalA1: Web of Science-articlepeer-review


BACKGROUND: SARS-CoV-2 vaccination is strongly recommended in kidney transplant recipients (KTR) and dialysis patients. Whether these vaccinations may trigger alloantibodies, is still debated.

METHODS: In the current study we evaluated the effect of SARS-CoV-2 mRNA vaccines on anti-Human Leukocyte Antigen (HLA) and 60 anti-non-HLA antibody profiles in clinically stable KTR and dialysis patients. In total, we included 28 KTR, 30 patients on haemodialysis, 25 patients on peritoneal dialysis and 31 controls with a positive seroresponse 16-21 days after the first dose of either the SARS-CoV-2 mRNA BNT162b2 or mRNA-1273 vaccine. Both anti-HLA and anti-non-HLA antibodies were determined prior to vaccination and 21 to 35 days after the second vaccine dose.

RESULTS: Overall, the proportion of patients with detectable anti-HLA antibodies was similar before and after vaccination (class I 14% vs. 16%, p = 0.48; class II 25% before and after vaccination). After vaccination, there was no pattern in 1) additionally detected anti-HLA antibodies, or 2) the levels of pre-existing ones. Additional anti-non-HLA antibodies were detected in 30% of the patients, ranging from 1 to 5 new anti-non-HLA antibodies per patient. However, the clinical significance of anti-non-HLA antibodies is still a matter of debate. To date, only a significant association has been found for anti-non-HLA ARHGDIB antibodies and long-term kidney graft loss. No additionally developed anti-ARHGDIB antibodies or elevated level of existing anti-ARHGDIB antibodies was observed.

CONCLUSION: The current data indicate that SARS-CoV-2 mRNA vaccination does not induce anti-HLA or anti-non-HLA antibodies, corroborating the importance of vaccinating KTR and dialysis patients.

Original languageEnglish
Article number101670
JournalTransplant immunology
Number of pages7
Publication statusPublished - 2022


  • 2019-nCoV Vaccine mRNA-1273
  • Antibodies, Viral
  • BNT162 Vaccine
  • COVID-19 Vaccines
  • COVID-19/prevention & control
  • Graft Rejection
  • HLA Antigens/genetics
  • Histocompatibility Antigens
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Humans
  • RNA, Messenger
  • Renal Dialysis
  • SARS-CoV-2
  • Vaccination
  • rho Guanine Nucleotide Dissociation Inhibitor beta


Dive into the research topics of 'SARS-CoV-2 mRNA vaccination is not associated with the induction of anti-HLA or non-HLA antibodies'. Together they form a unique fingerprint.

Cite this