Screening of strains of the Candida parapsilosis group of the BCCM/IHEM collection by MALDI-TOF MS

M Hendrickx; JS Goffinet; D Swinne; M Detandt

doi:10.1016/j.diagmicrobio.2011.04.006

Screening of strains of the Candida parapsilosis group of the BCCM/IHEM collection by MALDI-TOF MS

M Hendrickx, JS Goffinet, D Swinne, M Detandt

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

One hundred sixty-three strains stored as Candida parapsilosis in the BCCM/IHEM collection were reidentified based on internal transcribed spacer sequencing: 92% were identified as true C. parapsilosis, while 4.3% and 3% belonged to the closely related species C. metapsilosis and C. orthopsilosis, respectively, providing important epidemiologic information. Furthermore, we showed that matrix-assisted laser desorption ionisation time-of-flight mass spectrometry is a fast method that can discriminate between these species.

Original language	English
Journal	Diagnostic Microbiology and Infectious Disease
Volume	70
Issue number	4
Pages (from-to)	544-548
ISSN	0732-8893
DOIs	https://doi.org/10.1016/j.diagmicrobio.2011.04.006
Publication status	Published - 2011

Keywords

B780-tropical-medicine
Mycoses
Candidiasis
Candida parapsilosis
Candida metapsilosis
Candida orthopsilosis
Molecular epidemiology
Identification
Strains
Sequencing
Mass spectrometry

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10.1016/j.diagmicrobio.2011.04.006

http://lib.itg.be/pdf/itg/2011/2011dmid0544.pdf

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title = "Screening of strains of the Candida parapsilosis group of the BCCM/IHEM collection by MALDI-TOF MS",

abstract = "One hundred sixty-three strains stored as Candida parapsilosis in the BCCM/IHEM collection were reidentified based on internal transcribed spacer sequencing: 92% were identified as true C. parapsilosis, while 4.3% and 3% belonged to the closely related species C. metapsilosis and C. orthopsilosis, respectively, providing important epidemiologic information. Furthermore, we showed that matrix-assisted laser desorption ionisation time-of-flight mass spectrometry is a fast method that can discriminate between these species.",

keywords = "B780-tropical-medicine, Mycoses, Candidiasis, Candida parapsilosis, Candida metapsilosis, Candida orthopsilosis, Molecular epidemiology, Identification, Strains, Sequencing, Mass spectrometry",

author = "M Hendrickx and JS Goffinet and D Swinne and M Detandt",

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year = "2011",

doi = "10.1016/j.diagmicrobio.2011.04.006",

language = "English",

volume = "70",

pages = "544--548",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

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TY - JOUR

T1 - Screening of strains of the Candida parapsilosis group of the BCCM/IHEM collection by MALDI-TOF MS

AU - Hendrickx, M

AU - Goffinet, JS

AU - Swinne, D

AU - Detandt, M

N1 - NPP; ITG-X3C; OTHER; DOI; JIF; PDF; Abstract; CHAU

PY - 2011

Y1 - 2011

N2 - One hundred sixty-three strains stored as Candida parapsilosis in the BCCM/IHEM collection were reidentified based on internal transcribed spacer sequencing: 92% were identified as true C. parapsilosis, while 4.3% and 3% belonged to the closely related species C. metapsilosis and C. orthopsilosis, respectively, providing important epidemiologic information. Furthermore, we showed that matrix-assisted laser desorption ionisation time-of-flight mass spectrometry is a fast method that can discriminate between these species.

AB - One hundred sixty-three strains stored as Candida parapsilosis in the BCCM/IHEM collection were reidentified based on internal transcribed spacer sequencing: 92% were identified as true C. parapsilosis, while 4.3% and 3% belonged to the closely related species C. metapsilosis and C. orthopsilosis, respectively, providing important epidemiologic information. Furthermore, we showed that matrix-assisted laser desorption ionisation time-of-flight mass spectrometry is a fast method that can discriminate between these species.

KW - B780-tropical-medicine

KW - Mycoses

KW - Candidiasis

KW - Candida parapsilosis

KW - Candida metapsilosis

KW - Candida orthopsilosis

KW - Molecular epidemiology

KW - Identification

KW - Strains

KW - Sequencing

KW - Mass spectrometry

U2 - 10.1016/j.diagmicrobio.2011.04.006

DO - 10.1016/j.diagmicrobio.2011.04.006

M3 - A1: Web of Science-article

C2 - 21767712

VL - 70

SP - 544

EP - 548

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

IS - 4

ER -

Screening of strains of the Candida parapsilosis group of the BCCM/IHEM collection by MALDI-TOF MS

Abstract

Keywords

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