Sensitivity and kinetics of an NS1-based Zika virus enzyme-linked immunosorbent assay in Zika virus-infected travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile

Yaniv Lustig, Hana Zelena, Giulietta Venturi, Marjan Van Esbroeck, Camilla Rothe, Cecilia Perret, Ravit Koren, Shiri Katz-Likvornik, Ella Mendelson, Eli Schwartz

    Research output: Contribution to journalA1: Web of Science-article

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    Abstract

    Serological diagnosis of Zika virus is challenging due to high cross-reactivity of Zika virus with other flavivirus antibodies. Recently, a Zika NS1-based enzyme-linked immunosorbent assay (ELISA) was developed and shown to be highly specific for Zika antibody detection; however, sensitivity was evaluated for only a small number of confirmed Zika-infected patients. In this study, we measured the sensitivity and kinetics of Zika IgM and IgG antibodies using the Zika NS1-based ELISA in 105 samples from 63 returning travelers infected with Zika virus (proven by PCR or neutralization assay) from Israel, Czech Republic, Italy, Belgium, Germany, and Chile. Zika virus IgM was detected from 2 to 42 days post-symptom onset (PSO) with an overall sensitivity of 79% in the first month and 68% until 2 months PSO, while IgG antibodies were detected from 5 days to 3 years PSO with 79% sensitivity. Interestingly, significant differences in IgM sensitivity and IgM detection period were observed between Israeli and European/Chilean Zika-infected travelers, adding to the complexity of Zika infection diagnosis and suggesting that other diagnostic methods should be complemented to reduce false-negative results.

    Original languageEnglish
    JournalJournal of Clinical Microbiology
    Volume55
    Issue number6
    Pages (from-to)1894-1901
    Number of pages8
    ISSN0095-1137
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Journal Article

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