Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

FL Basiye; M Mbuchi; C Magiri; G Kirigi; S Deborggraeve; GJ Schoone; AA Saad; S El-Safi; E Matovu; MK Wasunna

doi:10.1111/j.1365-3156.2010.02548.x

Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

FL Basiye, M Mbuchi, C Magiri, G Kirigi, S Deborggraeve, GJ Schoone, AA Saad, S El-Safi, E Matovu, MK Wasunna

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Summary Objective To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively. Conclusion Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease

Original language	English
Journal	Tropical Medicine and International Health
Volume	15
Issue number	7
Pages (from-to)	806-810
Number of pages	5
ISSN	1360-2276
DOIs	https://doi.org/10.1111/j.1365-3156.2010.02548.x
Publication status	Published - 2010

Keywords

B780-tropical-medicine
Protozoal diseases
Leishmaniasis
Visceral
Leishmania
Vectors
Sandflies
Phlebotomus
Diagnosis
Molecular diagnostic techniques
Oligochromatography
NASBA
Assays
Sensitivity
Specificity
Markers
Laboratory techniques and procedures
Kenya
Africa-East

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Basiye, FL., Mbuchi, M., Magiri, C., Kirigi, G., Deborggraeve, S., Schoone, GJ., Saad, AA., El-Safi, S., Matovu, E., & Wasunna, MK. (2010). Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya. Tropical Medicine and International Health, 15(7), 806-810. https://doi.org/10.1111/j.1365-3156.2010.02548.x

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title = "Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya",

abstract = "Summary Objective To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively. Conclusion Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease",

keywords = "B780-tropical-medicine, Protozoal diseases, Leishmaniasis, Visceral, Leishmania, Vectors, Sandflies, Phlebotomus, Diagnosis, Molecular diagnostic techniques, Oligochromatography, NASBA, Assays, Sensitivity, Specificity, Markers, Laboratory techniques and procedures, Kenya, Africa-East",

author = "FL Basiye and M Mbuchi and C Magiri and G Kirigi and S Deborggraeve and GJ Schoone and AA Saad and S El-Safi and E Matovu and MK Wasunna",

note = "ITG-P5A; PARAS; U-SEROL; JIF; DOI; PDF; DSPACE; Abstract",

year = "2010",

doi = "10.1111/j.1365-3156.2010.02548.x",

language = "English",

volume = "15",

pages = "806--810",

journal = "Tropical Medicine and International Health",

issn = "1360-2276",

publisher = "John Wiley & Sons",

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Basiye, FL, Mbuchi, M, Magiri, C, Kirigi, G, Deborggraeve, S, Schoone, GJ, Saad, AA, El-Safi, S, Matovu, E & Wasunna, MK 2010, 'Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya', Tropical Medicine and International Health, vol. 15, no. 7, pp. 806-810. https://doi.org/10.1111/j.1365-3156.2010.02548.x

TY - JOUR

T1 - Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

AU - Basiye, FL

AU - Mbuchi, M

AU - Magiri, C

AU - Kirigi, G

AU - Deborggraeve, S

AU - Schoone, GJ

AU - Saad, AA

AU - El-Safi, S

AU - Matovu, E

AU - Wasunna, MK

N1 - ITG-P5A; PARAS; U-SEROL; JIF; DOI; PDF; DSPACE; Abstract

PY - 2010

Y1 - 2010

N2 - Summary Objective To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively. Conclusion Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease

AB - Summary Objective To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively. Conclusion Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Leishmaniasis

KW - Visceral

KW - Leishmania

KW - Vectors

KW - Sandflies

KW - Phlebotomus

KW - Diagnosis

KW - Molecular diagnostic techniques

KW - Oligochromatography

KW - NASBA

KW - Assays

KW - Sensitivity

KW - Specificity

KW - Markers

KW - Laboratory techniques and procedures

KW - Kenya

KW - Africa-East

U2 - 10.1111/j.1365-3156.2010.02548.x

DO - 10.1111/j.1365-3156.2010.02548.x

M3 - A1: Web of Science-article

C2 - 20487428

SN - 1360-2276

VL - 15

SP - 806

EP - 810

JO - Tropical Medicine and International Health

JF - Tropical Medicine and International Health

IS - 7

ER -

Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

Abstract

Keywords

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