Sensitivity and specificity of the Leishmania OligoC-TesT and NASBA-oligochromatography for diagnosis of visceral leishmaniasis in Kenya

FL Basiye, M Mbuchi, C Magiri, G Kirigi, S Deborggraeve, GJ Schoone, AA Saad, S El-Safi, E Matovu, MK Wasunna

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Summary Objective To estimate the sensitivity and specificity of the OligoC-TesT and nucleic acid sequence-based amplification coupled to oligochromatography (NASBA-OC) for molecular detection of Leishmania in blood from patients with confirmed visceral leishmaniasis (VL) and healthy endemic controls from Kenya. Methods Blood specimens of 84 patients with confirmed VL and 98 endemic healthy controls from Baringo district in Kenya were submitted to both assays. Results The Leishmania OligoC-TesT showed a sensitivity of 96.4% (95% confidence interval [CI]: 90-98.8%) and a specificity of 88.8% (95% CI: 81-93.6%), while the sensitivity and specificity of the NASBA-OC were 79.8% (95% CI: 67-87%) and 100% (95% CI: 96.3-100%), respectively. Conclusion Our findings indicate high sensitivity of the Leishmania OligoC-TesT on blood while the NASBA-OC is a better marker for active disease
    Original languageEnglish
    JournalTropical Medicine and International Health
    Volume15
    Issue number7
    Pages (from-to)806-810
    Number of pages5
    ISSN1360-2276
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Leishmaniasis
    • Visceral
    • Leishmania
    • Vectors
    • Sandflies
    • Phlebotomus
    • Diagnosis
    • Molecular diagnostic techniques
    • Oligochromatography
    • NASBA
    • Assays
    • Sensitivity
    • Specificity
    • Markers
    • Laboratory techniques and procedures
    • Kenya
    • Africa-East

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