Snow surface microbiome on the High Antarctic Plateau (DOME C)

Luigi Michaud, Angelina Lo Giudice, Mohamed Mysara, Pieter Monsieurs, Carmela Raffa, Natalie Leys, Stefano Amalfitano, Rob Van Houdt

Research output: Contribution to journalA1: Web of Science-article

Abstract

The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth's biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In this study, we investigated the microbial diversity in the surface snow surrounding the Concordia Research Station on the High Antarctic Plateau through a polyphasic approach, including direct prokaryotic quantification by flow cytometry and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), and phylogenetic identification by 16S RNA gene clone library sequencing and 454 16S amplicon pyrosequencing. Although the microbial abundance was low (<10(3) cells/ml of snowmelt), concordant results were obtained with the different techniques. The microbial community was mainly composed of members of the Alpha-proteobacteria class (e.g. Kiloniellaceae and Rhodobacteraceae), which is one of the most well-represented bacterial groups in marine habitats, Bacteroidetes (e.g. Cryomorphaceae and Flavobacteriaceae) and Cyanobacteria. Based on our results, polar microorganisms could not only be considered as deposited airborne particles, but as an active component of the snowpack ecology of the High Antarctic Plateau.

Original languageEnglish
JournalPLoS ONE
Volume9
Issue number8
Pages (from-to)e104505
ISSN1932-6203
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Antarctic Regions
  • Bacteria/classification
  • Biodiversity
  • Microbiota/physiology
  • RNA, Bacterial/genetics
  • RNA, Ribosomal, 16S/genetics

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