Some synonymous and nonsynonymous gyrA mutations in Mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the Hain GenoType MTBDRsl assays

Adebisi Ajileye, Nataly Alvarez, Matthias Merker, Timothy M. Walker, Suriya Akter, Kerstin Brown, Danesh Moradigaravand, Thomas Schoen, Soenke Andres, Viola Schleusener, Shaheed V. Omar, Francesc Coll, Hairong Huang, Roland Diel, Nazir Ismail, Julian Parkhill, Bouke C. de Jong, Tim E. A. Peto, Derrick W. Crook, Stefan NiemannJaime Robledo, E. Grace Smith, Sharon J. Peacock, Claudio U. Koeser

Research output: Contribution to journalA1: Web of Science-article

Abstract

In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.

Original languageEnglish
Article numberUNSP e02169-16
JournalAntimicrobial Agents and Chemotherapy
Volume61
Issue number4
Number of pages8
ISSN0066-4804
DOIs
Publication statusPublished - 2017

Keywords

  • Mycobacterium tuberculosis
  • Hain GenoType MTBDRsl
  • fluoroquinolones
  • LINE PROBE ASSAY
  • DRUG-RESISTANCE
  • ANTIBIOTIC-RESISTANCE
  • XDR TUBERCULOSIS
  • CLINICAL-SAMPLES
  • COMPLEX STRAINS
  • VERSION V2.0
  • PERFORMANCE
  • SUSCEPTIBILITY
  • 2ND-LINE

Cite this