Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections

Stijn Van Hees; Bart Cuypers; Stefan Bourgeois; Zwier M. A. Groothuismink; Pieter Meysman; Pieter Van der Vlies; Rob de Knegt; Luisa Vonghia; Peter Michielsen; Sven Francque; Kris Laukens; Andre Boonstra; Thomas Vanwolleghem

doi:10.1016/j.cellimm.2021.104283

Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections

Stijn Van Hees, Bart Cuypers, Stefan Bourgeois, Zwier M. A. Groothuismink, Pieter Meysman, Pieter Van der Vlies, Rob de Knegt, Luisa Vonghia, Peter Michielsen, Sven Francque, Kris Laukens, Andre Boonstra, Thomas Vanwolleghem

Molecular Parasitology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The natural course of chronic hepatitis B virus (HBV) infections follows distinct clinical disease phases, char-acterized by fluctuating levels of serum HBV DNA and ALT. The immune cells and their features that govern these clinical disease transitions remain unknown. In the current study, we performed RNA sequencing on pu-rified B cells from blood (n = 42) and liver (n = 10) of healthy controls and chronic HBV patients. We found distinct gene expression profiles between healthy controls and chronic HBV patients, as evidenced by 190 differentially expressed genes (DEG), but also between the clinical phenotypes of a chronic HBV infection (17?110 DEG between each phase). Numerous immune pathways, including the B cell receptor pathway were upregulated in liver B cells when compared to peripheral B cells. Further investigation of the detected DEG suggested an activation of B cells during HBeAg seroconversion and an active regulation of B cell signalling in the liver.

Original language	English
Article number	104283
Journal	Cellular Immunology
Volume	362
Number of pages	8
ISSN	0008-8749
DOIs	https://doi.org/10.1016/j.cellimm.2021.104283
Publication status	Published - 2021

Keywords

B cells
Hepatitis B
Activation
HBeAg seroconversion
Intrahepatic
RNA sequencing

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10.1016/j.cellimm.2021.104283

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Van Hees, S., Cuypers, B., Bourgeois, S., Groothuismink, Z. M. A., Meysman, P., Van der Vlies, P., de Knegt, R., Vonghia, L., Michielsen, P., Francque, S., Laukens, K., Boonstra, A., & Vanwolleghem, T. (2021). Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections. Cellular Immunology, 362, Article 104283. https://doi.org/10.1016/j.cellimm.2021.104283

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title = "Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections",

abstract = "The natural course of chronic hepatitis B virus (HBV) infections follows distinct clinical disease phases, char-acterized by fluctuating levels of serum HBV DNA and ALT. The immune cells and their features that govern these clinical disease transitions remain unknown. In the current study, we performed RNA sequencing on pu-rified B cells from blood (n = 42) and liver (n = 10) of healthy controls and chronic HBV patients. We found distinct gene expression profiles between healthy controls and chronic HBV patients, as evidenced by 190 differentially expressed genes (DEG), but also between the clinical phenotypes of a chronic HBV infection (17?110 DEG between each phase). Numerous immune pathways, including the B cell receptor pathway were upregulated in liver B cells when compared to peripheral B cells. Further investigation of the detected DEG suggested an activation of B cells during HBeAg seroconversion and an active regulation of B cell signalling in the liver.",

keywords = "B cells, Hepatitis B, Activation, HBeAg seroconversion, Intrahepatic, RNA sequencing",

author = "{Van Hees}, Stijn and Bart Cuypers and Stefan Bourgeois and Groothuismink, {Zwier M. A.} and Pieter Meysman and {Van der Vlies}, Pieter and {de Knegt}, Rob and Luisa Vonghia and Peter Michielsen and Sven Francque and Kris Laukens and Andre Boonstra and Thomas Vanwolleghem",

note = "NPP; OGOA",

year = "2021",

doi = "10.1016/j.cellimm.2021.104283",

language = "English",

volume = "362",

journal = "Cellular Immunology",

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Van Hees, S, Cuypers, B, Bourgeois, S, Groothuismink, ZMA, Meysman, P, Van der Vlies, P, de Knegt, R, Vonghia, L, Michielsen, P, Francque, S, Laukens, K, Boonstra, A & Vanwolleghem, T 2021, 'Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections', Cellular Immunology, vol. 362, 104283. https://doi.org/10.1016/j.cellimm.2021.104283

TY - JOUR

T1 - Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections

AU - Van Hees, Stijn

AU - Cuypers, Bart

AU - Bourgeois, Stefan

AU - Groothuismink, Zwier M. A.

AU - Meysman, Pieter

AU - Van der Vlies, Pieter

AU - de Knegt, Rob

AU - Vonghia, Luisa

AU - Michielsen, Peter

AU - Francque, Sven

AU - Laukens, Kris

AU - Boonstra, Andre

AU - Vanwolleghem, Thomas

N1 - NPP; OGOA

PY - 2021

Y1 - 2021

N2 - The natural course of chronic hepatitis B virus (HBV) infections follows distinct clinical disease phases, char-acterized by fluctuating levels of serum HBV DNA and ALT. The immune cells and their features that govern these clinical disease transitions remain unknown. In the current study, we performed RNA sequencing on pu-rified B cells from blood (n = 42) and liver (n = 10) of healthy controls and chronic HBV patients. We found distinct gene expression profiles between healthy controls and chronic HBV patients, as evidenced by 190 differentially expressed genes (DEG), but also between the clinical phenotypes of a chronic HBV infection (17?110 DEG between each phase). Numerous immune pathways, including the B cell receptor pathway were upregulated in liver B cells when compared to peripheral B cells. Further investigation of the detected DEG suggested an activation of B cells during HBeAg seroconversion and an active regulation of B cell signalling in the liver.

AB - The natural course of chronic hepatitis B virus (HBV) infections follows distinct clinical disease phases, char-acterized by fluctuating levels of serum HBV DNA and ALT. The immune cells and their features that govern these clinical disease transitions remain unknown. In the current study, we performed RNA sequencing on pu-rified B cells from blood (n = 42) and liver (n = 10) of healthy controls and chronic HBV patients. We found distinct gene expression profiles between healthy controls and chronic HBV patients, as evidenced by 190 differentially expressed genes (DEG), but also between the clinical phenotypes of a chronic HBV infection (17?110 DEG between each phase). Numerous immune pathways, including the B cell receptor pathway were upregulated in liver B cells when compared to peripheral B cells. Further investigation of the detected DEG suggested an activation of B cells during HBeAg seroconversion and an active regulation of B cell signalling in the liver.

KW - B cells

KW - Hepatitis B

KW - Activation

KW - HBeAg seroconversion

KW - Intrahepatic

KW - RNA sequencing

U2 - 10.1016/j.cellimm.2021.104283

DO - 10.1016/j.cellimm.2021.104283

M3 - A1: Web of Science-article

SN - 0008-8749

VL - 362

JO - Cellular Immunology

JF - Cellular Immunology

M1 - 104283

ER -

Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections

Abstract

Keywords

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