Spliced-Leader RNA as a dynamic marker for monitoring viable Leishmania parasites during and after treatment

R Hendrickx; R Melkamu; D Tadesse; T Teferi; PB Feijens; M Vleminckx; S van Henten; F Alves; T Shibru; J van Griensven; G Caljon; M Pareyn

doi:10.1093/infdis/jiae219

Spliced-Leader RNA as a dynamic marker for monitoring viable Leishmania parasites during and after treatment

R Hendrickx, R Melkamu, D Tadesse, T Teferi, PB Feijens, M Vleminckx, S van Henten, F Alves, T Shibru, J van Griensven, G Caljon, M Pareyn

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Accurate detection of viable Leishmania parasites is critical for evaluating visceral leishmaniasis (VL) treatment response at an early timepoint. We compared the decay of kinetoplast DNA (kDNA) and spliced-leader RNA (SL-RNA) in vitro, in vivo, and in a VL patient cohort. An optimized combination of blood preservation and nucleic acid extraction improved efficiency for both targets. SL-RNA degraded more rapidly during treatment than kDNA, and correlated better with microscopic examination. SL-RNA quantitative polymerase chain reaction emerges as a superior method for dynamic monitoring of viable Leishmania parasites. It enables individualized treatment monitoring for improved prognoses and has potential as an early surrogate endpoint in clinical trials.

Original language	English
Journal	Journal of Infectious Diseases
Number of pages	5
ISSN	0022-1899
DOIs	https://doi.org/10.1093/infdis/jiae219
Publication status	E-pub ahead of print - 2024

Keywords

PCR
Diagnostics
Leishmaniasis
Surrogate marker
Treatment response

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Hendrickx, R., Melkamu, R., Tadesse, D., Teferi, T., Feijens, PB., Vleminckx, M., van Henten, S., Alves, F., Shibru, T., van Griensven, J., Caljon, G., & Pareyn, M. (2024). Spliced-Leader RNA as a dynamic marker for monitoring viable Leishmania parasites during and after treatment. Journal of Infectious Diseases. Advance online publication. https://doi.org/10.1093/infdis/jiae219

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abstract = "Accurate detection of viable Leishmania parasites is critical for evaluating visceral leishmaniasis (VL) treatment response at an early timepoint. We compared the decay of kinetoplast DNA (kDNA) and spliced-leader RNA (SL-RNA) in vitro, in vivo, and in a VL patient cohort. An optimized combination of blood preservation and nucleic acid extraction improved efficiency for both targets. SL-RNA degraded more rapidly during treatment than kDNA, and correlated better with microscopic examination. SL-RNA quantitative polymerase chain reaction emerges as a superior method for dynamic monitoring of viable Leishmania parasites. It enables individualized treatment monitoring for improved prognoses and has potential as an early surrogate endpoint in clinical trials.",

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AU - Hendrickx, R

AU - Melkamu, R

AU - Tadesse, D

AU - Teferi, T

AU - Feijens, PB

AU - Vleminckx, M

AU - van Henten, S

AU - Alves, F

AU - Shibru, T

AU - van Griensven, J

AU - Caljon, G

AU - Pareyn, M

N1 - PPU;

PY - 2024

Y1 - 2024

N2 - Accurate detection of viable Leishmania parasites is critical for evaluating visceral leishmaniasis (VL) treatment response at an early timepoint. We compared the decay of kinetoplast DNA (kDNA) and spliced-leader RNA (SL-RNA) in vitro, in vivo, and in a VL patient cohort. An optimized combination of blood preservation and nucleic acid extraction improved efficiency for both targets. SL-RNA degraded more rapidly during treatment than kDNA, and correlated better with microscopic examination. SL-RNA quantitative polymerase chain reaction emerges as a superior method for dynamic monitoring of viable Leishmania parasites. It enables individualized treatment monitoring for improved prognoses and has potential as an early surrogate endpoint in clinical trials.

AB - Accurate detection of viable Leishmania parasites is critical for evaluating visceral leishmaniasis (VL) treatment response at an early timepoint. We compared the decay of kinetoplast DNA (kDNA) and spliced-leader RNA (SL-RNA) in vitro, in vivo, and in a VL patient cohort. An optimized combination of blood preservation and nucleic acid extraction improved efficiency for both targets. SL-RNA degraded more rapidly during treatment than kDNA, and correlated better with microscopic examination. SL-RNA quantitative polymerase chain reaction emerges as a superior method for dynamic monitoring of viable Leishmania parasites. It enables individualized treatment monitoring for improved prognoses and has potential as an early surrogate endpoint in clinical trials.

KW - PCR

KW - Diagnostics

KW - Leishmaniasis

KW - Surrogate marker

KW - Treatment response

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Spliced-Leader RNA as a dynamic marker for monitoring viable Leishmania parasites during and after treatment

Abstract

Keywords

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