Spontaneous mutation in the AgrRS two-component regulatory system of Cupriavidus metallidurans results in enhanced silver resistance

Kristel Mijnendonckx, Md Muntasir Ali, Ann Provoost, Paul Janssen, Max Mergeay, Natalie Leys, Daniel Charlier, Pieter Monsieurs, Rob Van Houdt

Research output: Contribution to journalA1: Web of Science-article

Abstract

The uncontrolled and widespread use of (nano)silver compounds has led to the increased release of these compounds into the environment, raising concerns about their negative impact on ecosystems. Concomitantly, silver resistance determinants are widely spread among environmental and clinically relevant bacteria although the underlying mechanisms are not yet fully understood. We show that Cupriavidus metallidurans is able to adapt to toxic silver concentrations. However, none of the known silver resistance determinants present in C. metallidurans are involved in the adaptative response. Instead, increased silver resistance is achieved by the concerted action of a two-component system AgrR-AgrS, previously not associated with metal resistance, and two periplasmic proteins PrsQ(1) and PrsQ(2). Both proteins belong to an unique group of small, uncharacterized, secreted proteins restricted to the genera Cupriavidus and Ralstonia. This system gives C. metallidurans the ability to withstand much higher silver concentrations. The latter could be facilitated by the accumulation of silver ions and the formation of silver nanoparticles.

Original languageEnglish
JournalMetallomics : Integrated Biometal Science
Volume11
Issue number11
Pages (from-to)1912-1924
Number of pages13
ISSN1756-5901
DOIs
Publication statusPublished - 1-Nov-2019
Externally publishedYes

Keywords

  • BROAD-HOST-RANGE
  • MICROBIAL CHARACTERIZATION
  • RALSTONIA-METALLIDURANS
  • RESPONSE REGULATOR
  • CLONING VECTORS
  • METAL
  • NANOPARTICLES
  • BACTERIA
  • EXPRESSION
  • PROTEIN

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