Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients

Benjamin J. Garcia, Andre G. Loxton, Gregory M. Dolganov, Tran T. Van, J. Lucian Davis, Bouke C. de Jong, Martin I. Voskuil, Sonia M. Leach, Gary K. Schoolnik, Gerhard Walzl, Michael Strong, Nicholas D. Walter

Research output: Contribution to journalA1: Web of Science-article

Abstract

Pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of Mycobacterium tuberculosis (M. tuberculosis) during infection and treatment. However, whether M. tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain. We therefore compared M. tuberculosis transcription in human sputum and bronchoalveolar lavage (BAL) samples from 11 HIV-negative South African patients with pulmonary tuberculosis. We additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic non-replicating persistence (NRP-2). Of 2179 M. tuberculosis transcripts assayed in sputum and BAL via multiplex RT-PCR, 194 (8.9%) had a p-value

Original languageEnglish
JournalTuberculosis
Volume100
Pages (from-to)89-94
Number of pages6
ISSN1472-9792
DOIs
Publication statusPublished - 2016

Keywords

  • Mycobacterium tuberculosis/genetics
  • Mycobacterium tuberculosis/physiology
  • Sputum/microbiology
  • Bronchoalveolar lavage/microbiology
  • Computational biology/methods
  • * Gene expression profiling
  • Tuberculosis
  • pulmonary/epidemiology/*microbiology
  • GENE-EXPRESSION
  • ADAPTATION
  • PERSISTENCE

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