Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients

Benjamin J. Garcia; Andre G. Loxton; Gregory M. Dolganov; Tran T. Van; J. Lucian Davis; Bouke C. de Jong; Martin I. Voskuil; Sonia M. Leach; Gary K. Schoolnik; Gerhard Walzl; Michael Strong; Nicholas D. Walter

doi:10.1016/j.tube.2016.07.004

Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients

Benjamin J. Garcia, Andre G. Loxton, Gregory M. Dolganov, Tran T. Van, J. Lucian Davis, Bouke C. de Jong, Martin I. Voskuil, Sonia M. Leach, Gary K. Schoolnik, Gerhard Walzl, Michael Strong, Nicholas D. Walter

Mycobacteriology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of Mycobacterium tuberculosis (M. tuberculosis) during infection and treatment. However, whether M. tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain. We therefore compared M. tuberculosis transcription in human sputum and bronchoalveolar lavage (BAL) samples from 11 HIV-negative South African patients with pulmonary tuberculosis. We additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic non-replicating persistence (NRP-2). Of 2179 M. tuberculosis transcripts assayed in sputum and BAL via multiplex RT-PCR, 194 (8.9%) had a p-value

Original language	English
Journal	Tuberculosis
Volume	100
Pages (from-to)	89-94
Number of pages	6
ISSN	1472-9792
DOIs	https://doi.org/10.1016/j.tube.2016.07.004
Publication status	Published - 2016

Keywords

Mycobacterium tuberculosis/genetics
Mycobacterium tuberculosis/physiology
Sputum/microbiology
Bronchoalveolar lavage/microbiology
Computational biology/methods
* Gene expression profiling
Tuberculosis
pulmonary/epidemiology/*microbiology
GENE-EXPRESSION
ADAPTATION
PERSISTENCE

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Garcia, B. J., Loxton, A. G., Dolganov, G. M., Van, T. T., Davis, J. L., de Jong, B. C., Voskuil, M. I., Leach, S. M., Schoolnik, G. K., Walzl, G., Strong, M., & Walter, N. D. (2016). Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients. Tuberculosis , 100, 89-94. https://doi.org/10.1016/j.tube.2016.07.004

@article{998955b6340a4ecdad53ad32aede5faa,

title = "Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients",

abstract = "Pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of Mycobacterium tuberculosis (M. tuberculosis) during infection and treatment. However, whether M. tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain. We therefore compared M. tuberculosis transcription in human sputum and bronchoalveolar lavage (BAL) samples from 11 HIV-negative South African patients with pulmonary tuberculosis. We additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic non-replicating persistence (NRP-2). Of 2179 M. tuberculosis transcripts assayed in sputum and BAL via multiplex RT-PCR, 194 (8.9%) had a p-value",

keywords = "Mycobacterium tuberculosis/genetics, Mycobacterium tuberculosis/physiology, Sputum/microbiology, Bronchoalveolar lavage/microbiology, Computational biology/methods, * Gene expression profiling, Tuberculosis, pulmonary/epidemiology/*microbiology, GENE-EXPRESSION, ADAPTATION, PERSISTENCE",

author = "Garcia, {Benjamin J.} and Loxton, {Andre G.} and Dolganov, {Gregory M.} and Van, {Tran T.} and Davis, {J. Lucian} and {de Jong}, {Bouke C.} and Voskuil, {Martin I.} and Leach, {Sonia M.} and Schoolnik, {Gary K.} and Gerhard Walzl and Michael Strong and Walter, {Nicholas D.}",

note = "NPP",

year = "2016",

doi = "10.1016/j.tube.2016.07.004",

language = "English",

volume = "100",

pages = "89--94",

journal = "Tuberculosis ",

issn = "1472-9792",

publisher = "Churchill Livingstone",

}

TY - JOUR

T1 - Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients

AU - Garcia, Benjamin J.

AU - Loxton, Andre G.

AU - Dolganov, Gregory M.

AU - Van, Tran T.

AU - Davis, J. Lucian

AU - de Jong, Bouke C.

AU - Voskuil, Martin I.

AU - Leach, Sonia M.

AU - Schoolnik, Gary K.

AU - Walzl, Gerhard

AU - Strong, Michael

AU - Walter, Nicholas D.

N1 - NPP

PY - 2016

Y1 - 2016

N2 - Pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of Mycobacterium tuberculosis (M. tuberculosis) during infection and treatment. However, whether M. tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain. We therefore compared M. tuberculosis transcription in human sputum and bronchoalveolar lavage (BAL) samples from 11 HIV-negative South African patients with pulmonary tuberculosis. We additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic non-replicating persistence (NRP-2). Of 2179 M. tuberculosis transcripts assayed in sputum and BAL via multiplex RT-PCR, 194 (8.9%) had a p-value

AB - Pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of Mycobacterium tuberculosis (M. tuberculosis) during infection and treatment. However, whether M. tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain. We therefore compared M. tuberculosis transcription in human sputum and bronchoalveolar lavage (BAL) samples from 11 HIV-negative South African patients with pulmonary tuberculosis. We additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic non-replicating persistence (NRP-2). Of 2179 M. tuberculosis transcripts assayed in sputum and BAL via multiplex RT-PCR, 194 (8.9%) had a p-value

KW - Mycobacterium tuberculosis/genetics

KW - Mycobacterium tuberculosis/physiology

KW - Sputum/microbiology

KW - Bronchoalveolar lavage/microbiology

KW - Computational biology/methods

KW - Gene expression profiling

KW - Tuberculosis

KW - pulmonary/epidemiology/microbiology

KW - GENE-EXPRESSION

KW - ADAPTATION

KW - PERSISTENCE

U2 - 10.1016/j.tube.2016.07.004

DO - 10.1016/j.tube.2016.07.004

M3 - A1: Web of Science-article

SN - 1472-9792

VL - 100

SP - 89

EP - 94

JO - Tuberculosis

JF - Tuberculosis

ER -

Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients

Abstract

Keywords

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