Stage determination in sleeping sickness: comparison of two cell counting and two parasite detection techniques

D. Mumba Ngoyi, J. Menten, P.P. Pyana, P. Büscher, V. Lejon

Research output: Contribution to journalA1: Web of Science-article

Abstract

OBJECTIVES: Diagnosis of the neurological stage of human African trypanosomiasis is performed by examination of cerebrospinal fluid (CSF) for the presence of trypanosomes and numbers of white blood cells (WBC). Both CSF parameters are also used to assess treatment outcome during follow-up. In view of the importance of CSF examination, and the practical problems encountered with it, we compared the sensitivity of two trypanosome concentration techniques and the repeatability of two cell counting methods, as well as occurrence of systematic differences between them. METHODS: Patients were recruited at Dipumba hospital, in Mbuji-Mayi in the Democratic Republic of the Congo. In 94 CSF samples, trypanosome detection was performed with modified single centrifugation (MSC) and double centrifugation (DC). On 189 CSF samples with
Original languageEnglish
JournalTropical Medicine and International Health
Volume18
Issue number6
Pages (from-to)778-782
Number of pages5
ISSN1360-2276
DOIs
Publication statusPublished - 2013

Keywords

  • Protozoal diseases
  • Sleeping sickness
  • Trypanosomiasis-African
  • Trypanosoma brucei gambiense
  • Vectors
  • Tsetse flies
  • Glossina morsitans
  • Disease progression
  • Stage determination
  • Cerebrospinal fluid
  • CSF
  • Parasite detection
  • Cell count
  • Centrifugation
  • Variability
  • Congo-Kinshasa
  • Africa-Central

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