Stage determination in sleeping sickness: comparison of two cell counting and two parasite detection techniques

D. Mumba Ngoyi; J. Menten; P.P. Pyana; P. Büscher; V. Lejon

doi:10.1111/tmi.12102

Stage determination in sleeping sickness: comparison of two cell counting and two parasite detection techniques

D. Mumba Ngoyi, J. Menten, P.P. Pyana, P. Büscher, V. Lejon

Clinical Trial Centre

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

OBJECTIVES: Diagnosis of the neurological stage of human African trypanosomiasis is performed by examination of cerebrospinal fluid (CSF) for the presence of trypanosomes and numbers of white blood cells (WBC). Both CSF parameters are also used to assess treatment outcome during follow-up. In view of the importance of CSF examination, and the practical problems encountered with it, we compared the sensitivity of two trypanosome concentration techniques and the repeatability of two cell counting methods, as well as occurrence of systematic differences between them. METHODS: Patients were recruited at Dipumba hospital, in Mbuji-Mayi in the Democratic Republic of the Congo. In 94 CSF samples, trypanosome detection was performed with modified single centrifugation (MSC) and double centrifugation (DC). On 189 CSF samples with

Original language	English
Journal	Tropical Medicine and International Health
Volume	18
Issue number	6
Pages (from-to)	778-782
Number of pages	5
ISSN	1360-2276
DOIs	https://doi.org/10.1111/tmi.12102
Publication status	Published - 2013

Keywords

Protozoal diseases
Sleeping sickness
Trypanosomiasis-African
Trypanosoma brucei gambiense
Vectors
Tsetse flies
Glossina morsitans
Disease progression
Stage determination
Cerebrospinal fluid
CSF
Parasite detection
Cell count
Centrifugation
Variability
Congo-Kinshasa
Africa-Central

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http://lib.itg.be/pdf/itg/2013/2013tmih0778.pdf

Cite this

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title = "Stage determination in sleeping sickness: comparison of two cell counting and two parasite detection techniques",

abstract = "OBJECTIVES: Diagnosis of the neurological stage of human African trypanosomiasis is performed by examination of cerebrospinal fluid (CSF) for the presence of trypanosomes and numbers of white blood cells (WBC). Both CSF parameters are also used to assess treatment outcome during follow-up. In view of the importance of CSF examination, and the practical problems encountered with it, we compared the sensitivity of two trypanosome concentration techniques and the repeatability of two cell counting methods, as well as occurrence of systematic differences between them. METHODS: Patients were recruited at Dipumba hospital, in Mbuji-Mayi in the Democratic Republic of the Congo. In 94 CSF samples, trypanosome detection was performed with modified single centrifugation (MSC) and double centrifugation (DC). On 189 CSF samples with ",

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author = "{Mumba Ngoyi}, D. and J. Menten and P.P. Pyana and P. B{\"u}scher and V. Lejon",

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doi = "10.1111/tmi.12102",

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TY - JOUR

T1 - Stage determination in sleeping sickness: comparison of two cell counting and two parasite detection techniques

AU - Mumba Ngoyi, D.

AU - Menten, J.

AU - Pyana, P.P.

AU - Büscher, P.

AU - Lejon, V.

N1 - NPP; ITG-C2A; ITG-B4A; ITG-BLA; MULTI; DCS; U-CTU; DBM; U-PARDIA; JIF; DOI; PDF; Abstract; DSPACE56

PY - 2013

Y1 - 2013

N2 - OBJECTIVES: Diagnosis of the neurological stage of human African trypanosomiasis is performed by examination of cerebrospinal fluid (CSF) for the presence of trypanosomes and numbers of white blood cells (WBC). Both CSF parameters are also used to assess treatment outcome during follow-up. In view of the importance of CSF examination, and the practical problems encountered with it, we compared the sensitivity of two trypanosome concentration techniques and the repeatability of two cell counting methods, as well as occurrence of systematic differences between them. METHODS: Patients were recruited at Dipumba hospital, in Mbuji-Mayi in the Democratic Republic of the Congo. In 94 CSF samples, trypanosome detection was performed with modified single centrifugation (MSC) and double centrifugation (DC). On 189 CSF samples with

AB - OBJECTIVES: Diagnosis of the neurological stage of human African trypanosomiasis is performed by examination of cerebrospinal fluid (CSF) for the presence of trypanosomes and numbers of white blood cells (WBC). Both CSF parameters are also used to assess treatment outcome during follow-up. In view of the importance of CSF examination, and the practical problems encountered with it, we compared the sensitivity of two trypanosome concentration techniques and the repeatability of two cell counting methods, as well as occurrence of systematic differences between them. METHODS: Patients were recruited at Dipumba hospital, in Mbuji-Mayi in the Democratic Republic of the Congo. In 94 CSF samples, trypanosome detection was performed with modified single centrifugation (MSC) and double centrifugation (DC). On 189 CSF samples with

KW - Protozoal diseases

KW - Sleeping sickness

KW - Trypanosomiasis-African

KW - Trypanosoma brucei gambiense

KW - Vectors

KW - Tsetse flies

KW - Glossina morsitans

KW - Disease progression

KW - Stage determination

KW - Cerebrospinal fluid

KW - CSF

KW - Parasite detection

KW - Cell count

KW - Centrifugation

KW - Variability

KW - Congo-Kinshasa

KW - Africa-Central

U2 - 10.1111/tmi.12102

DO - 10.1111/tmi.12102

M3 - A1: Web of Science-article

C2 - 23506188

SN - 1360-2276

VL - 18

SP - 778

EP - 782

JO - Tropical Medicine and International Health

JF - Tropical Medicine and International Health

IS - 6

ER -

Stage determination in sleeping sickness: comparison of two cell counting and two parasite detection techniques

Abstract

Keywords

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