Standardisation et évaluation de la technique de salivation manuelle pour le dépistage des infections par trypanosomes chez la glossine (Diptera: Glossinidae)

JM Kazadi, M Jochems, H Kabore, C Mbeng, J Van Hees, P Kageruka

Research output: Contribution to journalA2: International peer reviewed article (not A1-type)

Abstract

'Standardization and evaluation of a manual salivation method for the detection of Trypanosoma infection in the tsetse fly (Diptera: Glossinidae)': Two methods of salivation of tsetse flies, namely manual salivation and method of Bruce et al. were simultaneously evaluated on 1,702 male uninfected Glossina palpalis palpalis (Zaire), G. palpalis gambiensis (Bobo-Dioulasso), G. p. gambiensis (Maisons-Alfort) et G. morsitans morsitans (Mall) fasted for 23, 48 and 72 hours. The risk of salivation was 0.66 by the manual method and 0.01 by the method of Bruce et al. The manual salivation method was standardised on 79 male G. m. morsitans (Mall) infected with Trypanosoma congolense IL 1180. By this method, 70.88% of flies carrying mature and/or immature infection were identified. A clear difference was observed in the proportion of tsetse flies which salivated after 72 hours and those which salivated after 48 and 24 hours of fasting
Original languageFrench
JournalRevue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux
Volume48
Issue number2
Pages (from-to)171-175
ISSN0035-1865
Publication statusPublished - 1995

Keywords

  • B780-tropical-medicine
  • Protozoology
  • Trypanosoma congolense
  • Entomology
  • Tsetse flies
  • Glossina
  • Salivation
  • Laboratory medicine
  • Techniques

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