Targeted proteomics for studying pathogenic bacteria

Sara Saleh, An Staes, Stijn Deborggraeve, Kris Gevaert

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Mass spectrometry-based proteomics has been extensively used to map bacterial proteomes, which has led to a better understanding of the molecular mechanisms underlying bacterial infection and bacteria-host interactions. Quantitative proteomics using selected or parallel reaction monitoring is considered one of the most sensitive and specific quantitative MS-based approaches and has significantly advanced proteome studies of pathogenic bacteria. Here, recent applications of targeted proteomics for bacteria identification, biomarker discovery, and the characterization of bacterial virulence and antimicrobial resistance are reviewed among others. Results of such studies are expected to further contribute to improve the fight against the most common human pathogenic bacteria.

    Original languageEnglish
    Article number1800435
    JournalProteomics
    Number of pages10
    ISSN1615-9853
    DOIs
    Publication statusPublished - 2019

    Keywords

    • antibiotic resistance
    • bacteria
    • biomarkers
    • targeted proteomics
    • virulence
    • DATA-INDEPENDENT ACQUISITION
    • BACILLUS-ANTHRACIS SPORES
    • H BINDING-PROTEIN
    • MASS-SPECTROMETRY
    • ESCHERICHIA-COLI
    • MYCOBACTERIUM-TUBERCULOSIS
    • STREPTOCOCCUS-PYOGENES
    • STAPHYLOCOCCUS-AUREUS
    • HIGH-RESOLUTION
    • GLOBAL BURDEN

    Cite this