Tenofovir diphosphate and emtricitabine triphosphate concentrations in blood cells compared with isolated peripheral blood mononuclear cells: a new measure of antiretroviral adherence?

J.L. Adams; C. Sykes; P. Menezes; H.M. Prince; K.B. Patterson; K. Fransen; T. Crucitti; Irith De Baetselier; L. Van Damme; A.D. Kashuba

doi:10.1097/QAI.0b013e3182794723

Tenofovir diphosphate and emtricitabine triphosphate concentrations in blood cells compared with isolated peripheral blood mononuclear cells: a new measure of antiretroviral adherence?

J.L. Adams
, C. Sykes
, P. Menezes
, H.M. Prince
, K.B. Patterson
, K. Fransen
, T. Crucitti
, Irith De Baetselier
, L. Van Damme
, A.D. Kashuba

Research output: Contribution to journal › A1: Peer-reviewed journal articles › peer-review

Abstract

BACKGROUND: The active metabolites of tenofovir (TFV) and emtricitabine (FTC) in peripheral blood mononuclear cells (PBMCs) have been used as markers of long-term antiretroviral (ARV) adherence. However, the process of isolating PBMCs is expensive, complex, and not feasible in many settings. We compared concentrations of TFV-diphosphate (TFV-DP) and FTC-triphosphate (FTC-TP) in the upper layer packed cells (ULPC) obtained after whole blood centrifugation to isolated PBMCs as a possible alternative marker of adherence. METHODS:: Ten HIV+ adults with HIV RNA

Original language	English
Journal	Journal of Acquired Immune Deficiency Syndromes
Volume	62
Issue number	3
Pages (from-to)	260-266
Number of pages	7
ISSN	1525-4135
DOIs	https://doi.org/10.1097/QAI.0b013e3182794723
Publication status	Published - 2013

Keywords

Viral diseases
HIV
AIDS
HAART
Antiretrovirals
Compliance
Peripheral blood mononuclear cell (PBMC)
Markers
Concentration
Tenofovir
Emtricitabine
Liquid
Chromatography
Mass spectrometry
Correlation
Intracellular
Blood

Access to Document

10.1097/QAI.0b013e3182794723

http://lib.itg.be/pdf/itg/2013/2013jaid0260.pdf

Cite this

Adams, J. L., Sykes, C., Menezes, P., Prince, H. M., Patterson, K. B., Fransen, K., Crucitti, T., De Baetselier, I., Van Damme, L., & Kashuba, A. D. (2013). Tenofovir diphosphate and emtricitabine triphosphate concentrations in blood cells compared with isolated peripheral blood mononuclear cells: a new measure of antiretroviral adherence? Journal of Acquired Immune Deficiency Syndromes, 62(3), 260-266. https://doi.org/10.1097/QAI.0b013e3182794723

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title = "Tenofovir diphosphate and emtricitabine triphosphate concentrations in blood cells compared with isolated peripheral blood mononuclear cells: a new measure of antiretroviral adherence?",

abstract = "BACKGROUND: The active metabolites of tenofovir (TFV) and emtricitabine (FTC) in peripheral blood mononuclear cells (PBMCs) have been used as markers of long-term antiretroviral (ARV) adherence. However, the process of isolating PBMCs is expensive, complex, and not feasible in many settings. We compared concentrations of TFV-diphosphate (TFV-DP) and FTC-triphosphate (FTC-TP) in the upper layer packed cells (ULPC) obtained after whole blood centrifugation to isolated PBMCs as a possible alternative marker of adherence. METHODS:: Ten HIV+ adults with HIV RNA ",

keywords = "Viral diseases, HIV, AIDS, HAART, Antiretrovirals, Compliance, Peripheral blood mononuclear cell (PBMC), Markers, Concentration, Tenofovir, Emtricitabine, Liquid, Chromatography, Mass spectrometry, Correlation, Intracellular, Blood",

author = "J.L. Adams and C. Sykes and P. Menezes and H.M. Prince and K.B. Patterson and K. Fransen and T. Crucitti and \{De Baetselier\}, Irith and \{Van Damme\}, L. and A.D. Kashuba",

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year = "2013",

doi = "10.1097/QAI.0b013e3182794723",

language = "English",

volume = "62",

pages = "260--266",

journal = "Journal of Acquired Immune Deficiency Syndromes",

issn = "1525-4135",

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number = "3",

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Adams, JL, Sykes, C, Menezes, P, Prince, HM, Patterson, KB, Fransen, K, Crucitti, T, De Baetselier, I, Van Damme, L & Kashuba, AD 2013, 'Tenofovir diphosphate and emtricitabine triphosphate concentrations in blood cells compared with isolated peripheral blood mononuclear cells: a new measure of antiretroviral adherence?', Journal of Acquired Immune Deficiency Syndromes, vol. 62, no. 3, pp. 260-266. https://doi.org/10.1097/QAI.0b013e3182794723

Tenofovir diphosphate and emtricitabine triphosphate concentrations in blood cells compared with isolated peripheral blood mononuclear cells: a new measure of antiretroviral adherence? / Adams, J.L.; Sykes, C.; Menezes, P. et al.
In: Journal of Acquired Immune Deficiency Syndromes, Vol. 62, No. 3, 2013, p. 260-266.

Research output: Contribution to journal › A1: Peer-reviewed journal articles › peer-review