The Trypanosoma brucei TbHrg protein is a heme transporter involved in regulation of stage-specific morphological transitions

Eva Horáková, Piya Changmai, Marie Vancová, Roman Sobotka, Jan Van Den Abbeele, Benoit Vanhollebeke, Julius Lukeš

Research output: Contribution to journalA1: Web of Science-article

Abstract

The human parasite Trypanosoma brucei does not synthesize heme de novo and instead entirely relies on heme supplied by its vertebrate host or its insect vector, the tsetse fly. In the host bloodstream, T. brucei scavenges heme via haptoglobin-hemoglobin (HpHb) receptor-mediated endocytosis occurring in the flagellar pocket. However, in the procyclic developmental stage, in which T. brucei is confined to the tsetse fly midgut, this receptor is apparently not expressed, suggesting that T. brucei takes up heme by a different, unknown route. To define this alternative route, we functionally characterized heme transporter TbHrg in the procyclic stage. RNAi-induced down-regulation of TbHrg in heme-limited culture conditions resulted in slower proliferation, decreased cellular heme, and marked changes in cellular morphology so that the cells resemble mesocyclic trypomastigotes. Nevertheless, the TbHrg KO developed normally in the tsetse flies at rates comparable to the wild type cells. T. brucei cells overexpressing TbHrg displayed up-regulation of the early procyclin GPEET and down-regulation of the late procyclin EP1, two proteins coating the T. brucei surface in the procyclic stage. Light microscopy of immunostained TbHrg indicated localization to the flagellar membrane, and scanning electron microscopy revealed more intense TbHrg accumulation toward the flagellar pocket. Based on these findings, we postulate that T. brucei senses heme levels via the flagellar TbHrg protein. Heme deprivation in the tsetse fly anterior midgut might represent an environmental stimulus involved in the transformation of this important human parasite, possibly through metabolic remodeling.

Original languageEnglish
JournalJournal of Biological Chemistry
Volume292
Issue number17
Pages (from-to)6998-7010
Number of pages13
ISSN0021-9258
DOIs
Publication statusPublished - 2017

Keywords

  • Journal Article

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