The need for transparency and good practices in the qPCR literature

S.A. Bustin, V. Benes, J. Garson, J. Hellemans, J. Huggett, M. Kubista, R. Mueller, T. Nolan, M.W. Pfaffl, G. Shipley, C.T. Wittwer, P. Schjerling, P.J. Day, M. Abreu, B. Aguado, J.F. Beaulieu, A. Beckers, S. Bogaert, J.A. Browne, F. Carrasco-RamiroL. Ceelen, K. Ciborowski, P. Cornillie, S. Coulon, A. Cuypers, S. De Brouwer, L. De Ceuninck, J. De Craene, H. De Naeyer, W. De Spiegelaere, K. Deckers, A. Dheedene, K. Durinck, M. Ferreira-Teixeira, A. Fieuw, J.M. Gallup, S. Gonzalo-Flores, K. Goossens, F. Heindryckx, E. Herring, H. Hoenicka, L. Icardi, R. Jaggi, F. Javad, M. Karampelias, F. Kibenge, M. Kibenge, C. Kumps, I. Lambertz, T. Lammens, A. Markey, P. Messiaen, E. Mets, S. Morais, A. Mudarra-Rubio, J. Nakiwala, H. Nelis, P.A. Olsvik, C. Perez-Novo, M. Plusquin, T. Remans, A. Rihani, P. Rodrigues-Santos, P. Rondou, R. Sanders, K. Schmidt-Bleek, K. Skovgaard, K. Smeets, L. Tabera, S. Toegel, T. Van Acker, W. Van den Broeck, J. Van der Meulen, M. Van Gele, G. Van Peer, M. Van Poucke, N. Van Roy, S. Vergult, J. Wauman, M. Tshuikina-Wiklander, E. Willems, S. Zaccara, F. Zeka, J. Vandesompele

    Research output: Contribution to journalA1: Web of Science-articlepeer-review


    Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
    Original languageEnglish
    JournalNature Methods
    Issue number11
    Pages (from-to)1063-1067
    Number of pages5
    Publication statusPublished - 2013


    • Detection
    • Polymerase chain reaction
    • PCR
    • Information
    • Quantitative research
    • Guideline adherence
    • Journal impact factors
    • Scientific literature
    • Review of the literature


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