The need for transparency and good practices in the qPCR literature

S.A. Bustin; V. Benes; J. Garson; J. Hellemans; J. Huggett; M. Kubista; R. Mueller; T. Nolan; M.W. Pfaffl; G. Shipley; C.T. Wittwer; P. Schjerling; P.J. Day; M. Abreu; B. Aguado; J.F. Beaulieu; A. Beckers; S. Bogaert; J.A. Browne; F. Carrasco-Ramiro; L. Ceelen; K. Ciborowski; P. Cornillie; S. Coulon; A. Cuypers; S. De Brouwer; L. De Ceuninck; J. De Craene; H. De Naeyer; W. De Spiegelaere; K. Deckers; A. Dheedene; K. Durinck; M. Ferreira-Teixeira; A. Fieuw; J.M. Gallup; S. Gonzalo-Flores; K. Goossens; F. Heindryckx; E. Herring; H. Hoenicka; L. Icardi; R. Jaggi; F. Javad; M. Karampelias; F. Kibenge; M. Kibenge; C. Kumps; I. Lambertz; T. Lammens; A. Markey; P. Messiaen; E. Mets; S. Morais; A. Mudarra-Rubio; J. Nakiwala; H. Nelis; P.A. Olsvik; C. Perez-Novo; M. Plusquin; T. Remans; A. Rihani; P. Rodrigues-Santos; P. Rondou; R. Sanders; K. Schmidt-Bleek; K. Skovgaard; K. Smeets; L. Tabera; S. Toegel; T. Van Acker; W. Van den Broeck; J. Van der Meulen; M. Van Gele; G. Van Peer; M. Van Poucke; N. Van Roy; S. Vergult; J. Wauman; M. Tshuikina-Wiklander; E. Willems; S. Zaccara; F. Zeka; J. Vandesompele

doi:10.1038/nmeth.2697

The need for transparency and good practices in the qPCR literature

S.A. Bustin
, V. Benes
, J. Garson
, J. Hellemans
, J. Huggett
, M. Kubista
, R. Mueller
, T. Nolan
, M.W. Pfaffl
, G. Shipley
, C.T. Wittwer
, P. Schjerling
, P.J. Day
, M. Abreu
, B. Aguado
, J.F. Beaulieu
, A. Beckers
, S. Bogaert
, J.A. Browne
, F. Carrasco-Ramiro

L. Ceelen, K. Ciborowski, P. Cornillie, S. Coulon, A. Cuypers, S. De Brouwer, L. De Ceuninck, J. De Craene, H. De Naeyer, W. De Spiegelaere, K. Deckers, A. Dheedene, K. Durinck, M. Ferreira-Teixeira, A. Fieuw, J.M. Gallup, S. Gonzalo-Flores, K. Goossens, F. Heindryckx, E. Herring, H. Hoenicka, L. Icardi, R. Jaggi, F. Javad, M. Karampelias, F. Kibenge, M. Kibenge, C. Kumps, I. Lambertz, T. Lammens, A. Markey, P. Messiaen, E. Mets, S. Morais, A. Mudarra-Rubio, J. Nakiwala, H. Nelis, P.A. Olsvik, C. Perez-Novo, M. Plusquin, T. Remans, A. Rihani, P. Rodrigues-Santos, P. Rondou, R. Sanders, K. Schmidt-Bleek, K. Skovgaard, K. Smeets, L. Tabera, S. Toegel, T. Van Acker, W. Van den Broeck, J. Van der Meulen, M. Van Gele, G. Van Peer, M. Van Poucke, N. Van Roy, S. Vergult, J. Wauman, M. Tshuikina-Wiklander, E. Willems, S. Zaccara, F. Zeka, J. Vandesompele

Research output: Contribution to journal › A1: Peer-reviewed journal articles › peer-review

Abstract

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

Original language	English
Journal	Nature Methods
Volume	10
Issue number	11
Pages (from-to)	1063-1067
Number of pages	5
ISSN	1548-7091
DOIs	https://doi.org/10.1038/nmeth.2697
Publication status	Published - 2013

Keywords

Detection
Polymerase chain reaction
PCR
Information
Quantitative research
Guideline adherence
Journal impact factors
Scientific literature
Review of the literature

Access to Document

10.1038/nmeth.2697

http://www.ncbi.nlm.nih.gov/pubmed/24173381

Cite this

Bustin, S. A., Benes, V., Garson, J., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Shipley, G., Wittwer, C. T., Schjerling, P., Day, P. J., Abreu, M., Aguado, B., Beaulieu, J. F., Beckers, A., Bogaert, S., Browne, J. A., ... Vandesompele, J. (2013). The need for transparency and good practices in the qPCR literature. Nature Methods, 10(11), 1063-1067. https://doi.org/10.1038/nmeth.2697

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keywords = "Detection, Polymerase chain reaction, PCR, Information, Quantitative research, Guideline adherence, Journal impact factors, Scientific literature, Review of the literature",

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note = "NPP; ITG-B56B; DBM; U-IMMUN; JIF; DOI; Not available; DSPACE56",

year = "2013",

doi = "10.1038/nmeth.2697",

language = "English",

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journal = "Nature Methods",

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Bustin, SA, Benes, V, Garson, J, Hellemans, J, Huggett, J, Kubista, M, Mueller, R, Nolan, T, Pfaffl, MW, Shipley, G, Wittwer, CT, Schjerling, P, Day, PJ, Abreu, M, Aguado, B, Beaulieu, JF, Beckers, A, Bogaert, S, Browne, JA, Carrasco-Ramiro, F, Ceelen, L, Ciborowski, K, Cornillie, P, Coulon, S, Cuypers, A, De Brouwer, S, De Ceuninck, L, De Craene, J, De Naeyer, H, De Spiegelaere, W, Deckers, K, Dheedene, A, Durinck, K, Ferreira-Teixeira, M, Fieuw, A, Gallup, JM, Gonzalo-Flores, S, Goossens, K, Heindryckx, F, Herring, E, Hoenicka, H, Icardi, L, Jaggi, R, Javad, F, Karampelias, M, Kibenge, F, Kibenge, M, Kumps, C, Lambertz, I, Lammens, T, Markey, A, Messiaen, P, Mets, E, Morais, S, Mudarra-Rubio, A, Nakiwala, J, Nelis, H, Olsvik, PA, Perez-Novo, C, Plusquin, M, Remans, T, Rihani, A, Rodrigues-Santos, P, Rondou, P, Sanders, R, Schmidt-Bleek, K, Skovgaard, K, Smeets, K, Tabera, L, Toegel, S, Van Acker, T, Van den Broeck, W, Van der Meulen, J, Van Gele, M, Van Peer, G, Van Poucke, M, Van Roy, N, Vergult, S, Wauman, J, Tshuikina-Wiklander, M, Willems, E, Zaccara, S, Zeka, F & Vandesompele, J 2013, 'The need for transparency and good practices in the qPCR literature', Nature Methods, vol. 10, no. 11, pp. 1063-1067. https://doi.org/10.1038/nmeth.2697

TY - JOUR

T1 - The need for transparency and good practices in the qPCR literature

AU - Bustin, S.A.

AU - Benes, V.

AU - Garson, J.

AU - Hellemans, J.

AU - Huggett, J.

AU - Kubista, M.

AU - Mueller, R.

AU - Nolan, T.

AU - Pfaffl, M.W.

AU - Shipley, G.

AU - Wittwer, C.T.

AU - Schjerling, P.

AU - Day, P.J.

AU - Abreu, M.

AU - Aguado, B.

AU - Beaulieu, J.F.

AU - Beckers, A.

AU - Bogaert, S.

AU - Browne, J.A.

AU - Carrasco-Ramiro, F.

AU - Ceelen, L.

AU - Ciborowski, K.

AU - Cornillie, P.

AU - Coulon, S.

AU - Cuypers, A.

AU - De Brouwer, S.

AU - De Ceuninck, L.

AU - De Craene, J.

AU - De Naeyer, H.

AU - De Spiegelaere, W.

AU - Deckers, K.

AU - Dheedene, A.

AU - Durinck, K.

AU - Ferreira-Teixeira, M.

AU - Fieuw, A.

AU - Gallup, J.M.

AU - Gonzalo-Flores, S.

AU - Goossens, K.

AU - Heindryckx, F.

AU - Herring, E.

AU - Hoenicka, H.

AU - Icardi, L.

AU - Jaggi, R.

AU - Javad, F.

AU - Karampelias, M.

AU - Kibenge, F.

AU - Kibenge, M.

AU - Kumps, C.

AU - Lambertz, I.

AU - Lammens, T.

AU - Markey, A.

AU - Messiaen, P.

AU - Mets, E.

AU - Morais, S.

AU - Mudarra-Rubio, A.

AU - Nakiwala, J.

AU - Nelis, H.

AU - Olsvik, P.A.

AU - Perez-Novo, C.

AU - Plusquin, M.

AU - Remans, T.

AU - Rihani, A.

AU - Rodrigues-Santos, P.

AU - Rondou, P.

AU - Sanders, R.

AU - Schmidt-Bleek, K.

AU - Skovgaard, K.

AU - Smeets, K.

AU - Tabera, L.

AU - Toegel, S.

AU - Van Acker, T.

AU - Van den Broeck, W.

AU - Van der Meulen, J.

AU - Van Gele, M.

AU - Van Peer, G.

AU - Van Poucke, M.

AU - Van Roy, N.

AU - Vergult, S.

AU - Wauman, J.

AU - Tshuikina-Wiklander, M.

AU - Willems, E.

AU - Zaccara, S.

AU - Zeka, F.

AU - Vandesompele, J.

N1 - NPP; ITG-B56B; DBM; U-IMMUN; JIF; DOI; Not available; DSPACE56

PY - 2013

Y1 - 2013

N2 - Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

AB - Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

KW - Detection

KW - Polymerase chain reaction

KW - PCR

KW - Information

KW - Quantitative research

KW - Guideline adherence

KW - Journal impact factors

KW - Scientific literature

KW - Review of the literature

U2 - 10.1038/nmeth.2697

DO - 10.1038/nmeth.2697

M3 - A1: Peer-reviewed journal articles

C2 - 24173381

SN - 1548-7091

VL - 10

SP - 1063

EP - 1067

JO - Nature Methods

JF - Nature Methods

IS - 11

ER -

The need for transparency and good practices in the qPCR literature

Abstract

Keywords

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