The potential of metabolomics for Leishmania research in the post-genomics era

RA Scheltema, S Decuypere, Ruben t`Kindt, JC Dujardin, GH Coombs, R Breitling

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    SUMMARY: The post-genomics era has provided researchers with access to a new generation of tools for the global characterization and understanding of pathogen diversity. This review provides a critical summary of published Leishmania post-genomic research efforts to date, and discusses the potential impact of the addition of metabolomics to the post-genomic toolbox. Metabolomics aims at understanding biology by comprehensive metabolite profiling. We present an overview of the design and interpretation of metabolomics experiments in the context of Leishmania research. Sample preparation, measurement techniques, and bioinformatics analysis of the generated complex datasets are discussed in detail. To illustrate the concepts and the expected results of metabolomics analyses, we also present an overview of comparative metabolic profiles of drug-sensitive and drug-resistant Leishmania donovani clinical isolates
    Original languageEnglish
    JournalParasitology
    Volume137
    Issue number9
    Pages (from-to)1291-1302
    Number of pages12
    ISSN0031-1820
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Leishmaniasis
    • Leishmania
    • Leishmania donovani
    • Genomics
    • Mass spectrometry
    • Liquid
    • Chromatography
    • Pathogens
    • Diversity
    • Tools
    • Sampling
    • Preparations
    • Measurement
    • Metabolomics
    • Genome
    • Proteomics
    • Drug sensitivity
    • Drug resistance
    • Laboratory techniques and procedures
    • Review of the literature

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