The rrs (16S) - rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay

XX Gu; R Rossau; G Jannes; R Ballard; M Laga; E Van Dyck

doi:10.1099/00221287-144-4-1013

The rrs (16S) - rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay

XX Gu, R Rossau, G Jannes, R Ballard, M Laga, E Van Dyck

Sexual Health including HIV

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.

Original language	English
Journal	Microbiology
Volume	144
Issue number	4
Pages (from-to)	1013-1019
Number of pages	7
DOIs	https://doi.org/10.1099/00221287-144-4-1013
Publication status	Published - 1998

Keywords

B780-tropical-medicine
Bacterial diseases
Sexually transmitted diseases
STD
Chancroid
Haemophilus ducreyi
Laboratory medicine
Diagnosis
PCR

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10.1099/00221287-144-4-1013

http://lib.itg.be/pdf/itg/1998/1998micr1013.pdf

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title = "The rrs (16S) - rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay",

abstract = "The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.",

keywords = "B780-tropical-medicine, Bacterial diseases, Sexually transmitted diseases, STD, Chancroid, Haemophilus ducreyi, Laboratory medicine, Diagnosis, PCR",

author = "XX Gu and R Rossau and G Jannes and R Ballard and M Laga and {Van Dyck}, E",

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year = "1998",

doi = "10.1099/00221287-144-4-1013",

language = "English",

volume = "144",

pages = "1013--1019",

journal = "Microbiology",

publisher = "Society for General Microbiology",

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TY - JOUR

T1 - The rrs (16S) - rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay

AU - Gu, XX

AU - Rossau, R

AU - Jannes, G

AU - Ballard, R

AU - Laga, M

AU - Van Dyck, E

N1 - FTX: Abonnement

PY - 1998

Y1 - 1998

N2 - The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.

AB - The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.

KW - B780-tropical-medicine

KW - Bacterial diseases

KW - Sexually transmitted diseases

KW - STD

KW - Chancroid

KW - Haemophilus ducreyi

KW - Laboratory medicine

KW - Diagnosis

KW - PCR

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000073186400022

U2 - 10.1099/00221287-144-4-1013

DO - 10.1099/00221287-144-4-1013

M3 - A1: Web of Science-article

VL - 144

SP - 1013

EP - 1019

JO - Microbiology

JF - Microbiology

IS - 4

ER -

The rrs (16S) - rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay

Abstract

Keywords

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