The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services

V Vincent; L Rigouts; E Nduwamahoro; B Holmes; J Cunningham; M Guillerm; CM Nathanson; F Moussy; B De Jong; F Portaels; A Ramsay

doi:10.5588/ijtld.11.0223

The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services

V Vincent, L Rigouts, E Nduwamahoro, B Holmes, J Cunningham, M Guillerm, CM Nathanson, F Moussy, B De Jong, F Portaels, A Ramsay

Mycobacteriology

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Abstract

BACKGROUND: The Special Programme for Research and Training in Tropical Diseases recently launched a Mycobacterium tuberculosis strain bank (TDR-TB Strain Bank). OBJECTIVE: To describe the TDR-TB Strain Bank, the characterisation of strains, bank management and the procedure for releasing materials. RESULTS: The TDR-TB Strain Bank consists of 229 clinical M. tuberculosis isolates (single-colony derived cultures) plus five mycobacterial reference strains for purposes of identification. These are available as freeze-dried, viable strains or as heat-inactivated bacterial suspensions, quality controlled for purity, viability and authenticity. Isolates originated from diverse geographical settings and were selected for their resistance profiles against first- and second-line drugs. Low and high levels of resistance were determined by the minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol, streptomycin, ofloxacin, kanamycin, capreomycin, ethionamide and para-aminosalicylic acid. Sequencing for drug resistance mutations was performed on the relevant sections of the rpoB, katG, inhA, embB, rpsL, rrs, gyrA and gyrB genes. Typing using lineage-defining loci of mycobacterial interspersed repetitive unit-variable number tandem repeats indicated that the most important genetic lineages were represented. CONCLUSIONS: The TDR-TB Strain Bank is a high quality bioresource for basic science, supporting the development of new diagnostics and drug-resistant detection tools and providing reference materials for laboratory quality management programmes.

Original language	English
Journal	International Journal of Tuberculosis and Lung Disease
Volume	16
Issue number	1
Pages (from-to)	24-31
ISSN	1027-3719
DOIs	https://doi.org/10.5588/ijtld.11.0223
Publication status	Published - 2012

Keywords

B780-tropical-medicine
Bacterial diseases
Tuberculosis
Mycobacterium tuberculosis
Research
Strains
Isolation
Culture collection
Specimen collection
Identification
Quality control
Drug resistance
Mutations
Genetic variation
Biotechnology
Institute of Tropical Medicine (ITM)
ITM
Belgium
Europe-West

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10.5588/ijtld.11.0223

2012ijtl0024

http://lib.itg.be/pdf/itg/2012/2012ijtl0024.pdf

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Vincent, V., Rigouts, L., Nduwamahoro, E., Holmes, B., Cunningham, J., Guillerm, M., Nathanson, CM., Moussy, F., De Jong, B., Portaels, F., & Ramsay, A. (2012). The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services. International Journal of Tuberculosis and Lung Disease, 16(1), 24-31. https://doi.org/10.5588/ijtld.11.0223

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title = "The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services",

abstract = "BACKGROUND: The Special Programme for Research and Training in Tropical Diseases recently launched a Mycobacterium tuberculosis strain bank (TDR-TB Strain Bank). OBJECTIVE: To describe the TDR-TB Strain Bank, the characterisation of strains, bank management and the procedure for releasing materials. RESULTS: The TDR-TB Strain Bank consists of 229 clinical M. tuberculosis isolates (single-colony derived cultures) plus five mycobacterial reference strains for purposes of identification. These are available as freeze-dried, viable strains or as heat-inactivated bacterial suspensions, quality controlled for purity, viability and authenticity. Isolates originated from diverse geographical settings and were selected for their resistance profiles against first- and second-line drugs. Low and high levels of resistance were determined by the minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol, streptomycin, ofloxacin, kanamycin, capreomycin, ethionamide and para-aminosalicylic acid. Sequencing for drug resistance mutations was performed on the relevant sections of the rpoB, katG, inhA, embB, rpsL, rrs, gyrA and gyrB genes. Typing using lineage-defining loci of mycobacterial interspersed repetitive unit-variable number tandem repeats indicated that the most important genetic lineages were represented. CONCLUSIONS: The TDR-TB Strain Bank is a high quality bioresource for basic science, supporting the development of new diagnostics and drug-resistant detection tools and providing reference materials for laboratory quality management programmes.",

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author = "V Vincent and L Rigouts and E Nduwamahoro and B Holmes and J Cunningham and M Guillerm and CM Nathanson and F Moussy and {De Jong}, B and F Portaels and A Ramsay",

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Vincent, V, Rigouts, L, Nduwamahoro, E, Holmes, B, Cunningham, J, Guillerm, M, Nathanson, CM, Moussy, F, De Jong, B, Portaels, F & Ramsay, A 2012, 'The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services', International Journal of Tuberculosis and Lung Disease, vol. 16, no. 1, pp. 24-31. https://doi.org/10.5588/ijtld.11.0223

TY - JOUR

T1 - The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services

AU - Vincent, V

AU - Rigouts, L

AU - Nduwamahoro, E

AU - Holmes, B

AU - Cunningham, J

AU - Guillerm, M

AU - Nathanson, CM

AU - Moussy, F

AU - De Jong, B

AU - Portaels, F

AU - Ramsay, A

N1 - FTX; ITG-B2A; ITG-B3B; ITG-B9A; ITG-X10B; DBM; U-MYCOB; JIF; DOI; PDF; Abstract; DSPACE

PY - 2012

Y1 - 2012

N2 - BACKGROUND: The Special Programme for Research and Training in Tropical Diseases recently launched a Mycobacterium tuberculosis strain bank (TDR-TB Strain Bank). OBJECTIVE: To describe the TDR-TB Strain Bank, the characterisation of strains, bank management and the procedure for releasing materials. RESULTS: The TDR-TB Strain Bank consists of 229 clinical M. tuberculosis isolates (single-colony derived cultures) plus five mycobacterial reference strains for purposes of identification. These are available as freeze-dried, viable strains or as heat-inactivated bacterial suspensions, quality controlled for purity, viability and authenticity. Isolates originated from diverse geographical settings and were selected for their resistance profiles against first- and second-line drugs. Low and high levels of resistance were determined by the minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol, streptomycin, ofloxacin, kanamycin, capreomycin, ethionamide and para-aminosalicylic acid. Sequencing for drug resistance mutations was performed on the relevant sections of the rpoB, katG, inhA, embB, rpsL, rrs, gyrA and gyrB genes. Typing using lineage-defining loci of mycobacterial interspersed repetitive unit-variable number tandem repeats indicated that the most important genetic lineages were represented. CONCLUSIONS: The TDR-TB Strain Bank is a high quality bioresource for basic science, supporting the development of new diagnostics and drug-resistant detection tools and providing reference materials for laboratory quality management programmes.

AB - BACKGROUND: The Special Programme for Research and Training in Tropical Diseases recently launched a Mycobacterium tuberculosis strain bank (TDR-TB Strain Bank). OBJECTIVE: To describe the TDR-TB Strain Bank, the characterisation of strains, bank management and the procedure for releasing materials. RESULTS: The TDR-TB Strain Bank consists of 229 clinical M. tuberculosis isolates (single-colony derived cultures) plus five mycobacterial reference strains for purposes of identification. These are available as freeze-dried, viable strains or as heat-inactivated bacterial suspensions, quality controlled for purity, viability and authenticity. Isolates originated from diverse geographical settings and were selected for their resistance profiles against first- and second-line drugs. Low and high levels of resistance were determined by the minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol, streptomycin, ofloxacin, kanamycin, capreomycin, ethionamide and para-aminosalicylic acid. Sequencing for drug resistance mutations was performed on the relevant sections of the rpoB, katG, inhA, embB, rpsL, rrs, gyrA and gyrB genes. Typing using lineage-defining loci of mycobacterial interspersed repetitive unit-variable number tandem repeats indicated that the most important genetic lineages were represented. CONCLUSIONS: The TDR-TB Strain Bank is a high quality bioresource for basic science, supporting the development of new diagnostics and drug-resistant detection tools and providing reference materials for laboratory quality management programmes.

KW - B780-tropical-medicine

KW - Bacterial diseases

KW - Tuberculosis

KW - Mycobacterium tuberculosis

KW - Research

KW - Strains

KW - Isolation

KW - Culture collection

KW - Specimen collection

KW - Identification

KW - Quality control

KW - Drug resistance

KW - Mutations

KW - Genetic variation

KW - Biotechnology

KW - Institute of Tropical Medicine (ITM)

KW - ITM

KW - Belgium

KW - Europe-West

U2 - 10.5588/ijtld.11.0223

DO - 10.5588/ijtld.11.0223

M3 - A1: Web of Science-article

C2 - 22236841

SN - 1027-3719

VL - 16

SP - 24

EP - 31

JO - International Journal of Tuberculosis and Lung Disease

JF - International Journal of Tuberculosis and Lung Disease

IS - 1

ER -

The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services

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