Transmission of bluetongue virus serotype 8 by artificial insemination with frozen-thawed semen from naturally infected bulls

Kris De Clercq, Leen Vandaele, Tine Vanbinst, Mickael Riou, Isra Deblauwe, Wendy Wesselingh, Anne Pinard, Mieke Van Eetvelde, Olivier Boulesteix, Bart Leemans, Robert Gelineau, Griet Vercauteren, Sara Van der Heyden, Jean-Francois Beckers, Claude Saegerman, Donal Sammin, Aart de Kruif, Ilse De Leeuw

Research output: Contribution to journalA1: Web of Science-article

Abstract

Transmission of bluetongue (BT) virus serotype 8 (BTV-8) via artificial insemination of contaminated frozen semen from naturally infected bulls was investigated in two independent experiments. Healthy, BT negative heifers were hormonally synchronized and artificially inseminated at oestrus. In total, six groups of three heifers received semen from four batches derived from three bulls naturally infected with BTV-8. Each experiment included one control heifer that was not inseminated and that remained BT negative throughout. BTV viraemia and seroconversion were determined in 8 out of 18 inseminated heifers, and BTV was isolated from five of these animals. These eight heifers only displayed mild clinical signs of BT, if any at all, but six of them experienced pregnancy loss between weeks four and eight of gestation, and five of them became BT PCR and antibody positive. The other two infected heifers gave birth at term to two healthy and BT negative calves. The BT viral load varied among the semen batches used and this had a significant impact on the infection rate, the time of onset of viraemia post artificial insemination, and the gestational stage at which pregnancy loss occurred. These results, which confirm unusual features of BTV-8 infection, should not be extrapolated to infection with other BTV strains without thorough evaluation. This study also adds weight to the hypothesis that the re-emergence of BTV-8 in France in 2015 may be attributable to the use of contaminated bovine semen.

Original languageEnglish
Article number652
JournalViruses-Basel
Volume13
Issue number4
Number of pages16
ISSN1999-4915
DOIs
Publication statusPublished - 2021

Keywords

  • bluetongue
  • bluetongue virus
  • serotype 8
  • BTV-8
  • transmission
  • artificial insemination
  • semen
  • cattle
  • TIME RT-PCR
  • CATTLE
  • BELGIUM
  • SHEEP
  • RADIOIMMUNOASSAY
  • PATHOGENESIS
  • REEMERGENCE
  • EPIDEMIC
  • CALVES
  • BLOOD

Cite this