Tuberculosis drug resistance testing by molecular methods: opportunities and challenges in resource limited settings

G Abebe; F Paasch; L Apers; L Rigouts; R Colebunders

doi:10.1016/j.mimet.2010.11.014

Tuberculosis drug resistance testing by molecular methods: opportunities and challenges in resource limited settings

G Abebe, F Paasch, L Apers, L Rigouts, R Colebunders

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

One of the greatest threats to global tuberculosis (TB) control is the growing prevalence of drug-resistant bacilli. Correctly diagnosing drug-resistant TB patients is more problematic in resource-limited settings as there is no or limited infrastructure for drug susceptibility testing (DST) of TB bacilli. The conventional phenotypic DST method for TB takes weeks before declaring the results and initiating proper anti-TB treatment. Molecular DST offers advantages over the phenotypic methods mainly because of the short turn around time. This review summarizes the different molecular DST methods for TB and discusses challenges and opportunities in implementing them in resource-limited settings.

Original language	English
Journal	Journal of Microbiological Methods
Volume	84
Issue number	2
Pages (from-to)	155-160
Number of pages	6
ISSN	0167-7012
DOIs	https://doi.org/10.1016/j.mimet.2010.11.014
Publication status	Published - 2011

Keywords

B780-tropical-medicine
Bacterial diseases
Tuberculosis
Mycobacterium tuberculosis
Diagnosis
Drug resistance
Drug susceptibility
Testing
Molecular
Real-time
Polymerase chain reaction
PCR
Solid phase cytometry
Hybridization assay
Sequencing
Barriers
Efficiency
Laboratory techniques and procedures
Review of the literature

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10.1016/j.mimet.2010.11.014

http://lib.itg.be/pdf/itg/2011/2011jmme0155.pdf

Cite this

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title = "Tuberculosis drug resistance testing by molecular methods: opportunities and challenges in resource limited settings",

abstract = "One of the greatest threats to global tuberculosis (TB) control is the growing prevalence of drug-resistant bacilli. Correctly diagnosing drug-resistant TB patients is more problematic in resource-limited settings as there is no or limited infrastructure for drug susceptibility testing (DST) of TB bacilli. The conventional phenotypic DST method for TB takes weeks before declaring the results and initiating proper anti-TB treatment. Molecular DST offers advantages over the phenotypic methods mainly because of the short turn around time. This review summarizes the different molecular DST methods for TB and discusses challenges and opportunities in implementing them in resource-limited settings.",

keywords = "B780-tropical-medicine, Bacterial diseases, Tuberculosis, Mycobacterium tuberculosis, Diagnosis, Drug resistance, Drug susceptibility, Testing, Molecular, Real-time, Polymerase chain reaction, PCR, Solid phase cytometry, Hybridization assay, Sequencing, Barriers, Efficiency, Laboratory techniques and procedures, Review of the literature",

author = "G Abebe and F Paasch and L Apers and L Rigouts and R Colebunders",

note = "NPP; ITG-C3A; ITG-M4A; ITG-CLA; MULTI; CLINIC; U-HIVCLI; MICRO; U-MYCOB; JIF; DOI; PDF; Abstract; DSPACE",

year = "2011",

doi = "10.1016/j.mimet.2010.11.014",

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T1 - Tuberculosis drug resistance testing by molecular methods: opportunities and challenges in resource limited settings

AU - Abebe, G

AU - Paasch, F

AU - Apers, L

AU - Rigouts, L

AU - Colebunders, R

N1 - NPP; ITG-C3A; ITG-M4A; ITG-CLA; MULTI; CLINIC; U-HIVCLI; MICRO; U-MYCOB; JIF; DOI; PDF; Abstract; DSPACE

PY - 2011

Y1 - 2011

N2 - One of the greatest threats to global tuberculosis (TB) control is the growing prevalence of drug-resistant bacilli. Correctly diagnosing drug-resistant TB patients is more problematic in resource-limited settings as there is no or limited infrastructure for drug susceptibility testing (DST) of TB bacilli. The conventional phenotypic DST method for TB takes weeks before declaring the results and initiating proper anti-TB treatment. Molecular DST offers advantages over the phenotypic methods mainly because of the short turn around time. This review summarizes the different molecular DST methods for TB and discusses challenges and opportunities in implementing them in resource-limited settings.

AB - One of the greatest threats to global tuberculosis (TB) control is the growing prevalence of drug-resistant bacilli. Correctly diagnosing drug-resistant TB patients is more problematic in resource-limited settings as there is no or limited infrastructure for drug susceptibility testing (DST) of TB bacilli. The conventional phenotypic DST method for TB takes weeks before declaring the results and initiating proper anti-TB treatment. Molecular DST offers advantages over the phenotypic methods mainly because of the short turn around time. This review summarizes the different molecular DST methods for TB and discusses challenges and opportunities in implementing them in resource-limited settings.

KW - B780-tropical-medicine

KW - Bacterial diseases

KW - Tuberculosis

KW - Mycobacterium tuberculosis

KW - Diagnosis

KW - Drug resistance

KW - Drug susceptibility

KW - Testing

KW - Molecular

KW - Real-time

KW - Polymerase chain reaction

KW - PCR

KW - Solid phase cytometry

KW - Hybridization assay

KW - Sequencing

KW - Barriers

KW - Efficiency

KW - Laboratory techniques and procedures

KW - Review of the literature

U2 - 10.1016/j.mimet.2010.11.014

DO - 10.1016/j.mimet.2010.11.014

M3 - A1: Web of Science-article

C2 - 21129417

SN - 0167-7012

VL - 84

SP - 155

EP - 160

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

Tuberculosis drug resistance testing by molecular methods: opportunities and challenges in resource limited settings

Abstract

Keywords

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