Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada

Carl Boodman, Courtney Loomer, Antonia Dibernardo, Todd Hatchette, Jason J LeBlanc, Brooks Waitt, L Robbin Lindsay

Research output: Contribution to journalArticlepeer-review

Abstract

Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%-95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable.

Original languageEnglish
JournalEmerging Infectious Diseases
Volume29
Issue number1
Pages (from-to)175-178
Number of pages4
ISSN1080-6040
DOIs
Publication statusPublished - Jan-2023

Keywords

  • Animals
  • Humans
  • Anaplasmosis/diagnosis
  • Real-Time Polymerase Chain Reaction
  • Anaplasma phagocytophilum/genetics
  • Canada/epidemiology

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