Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada

Carl Boodman; Courtney Loomer; Antonia Dibernardo; Todd Hatchette; Jason J LeBlanc; Brooks Waitt; L Robbin Lindsay

doi:10.3201/eid2901.220988

Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada

Carl Boodman, Courtney Loomer, Antonia Dibernardo, Todd Hatchette, Jason J LeBlanc, Brooks Waitt, L Robbin Lindsay

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%-95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable.

Original language	English
Journal	Emerging Infectious Diseases
Volume	29
Issue number	1
Pages (from-to)	175-178
Number of pages	4
ISSN	1080-6040
DOIs	https://doi.org/10.3201/eid2901.220988
Publication status	Published - Jan-2023
Externally published	Yes

Keywords

Animals
Humans
Anaplasmosis/diagnosis
Real-Time Polymerase Chain Reaction
Anaplasma phagocytophilum/genetics
Canada/epidemiology

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10.3201/eid2901.220988

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title = "Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada",

abstract = "Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%-95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable.",

keywords = "Animals, Humans, Anaplasmosis/diagnosis, Real-Time Polymerase Chain Reaction, Anaplasma phagocytophilum/genetics, Canada/epidemiology",

author = "Carl Boodman and Courtney Loomer and Antonia Dibernardo and Todd Hatchette and LeBlanc, {Jason J} and Brooks Waitt and Lindsay, {L Robbin}",

year = "2023",

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language = "English",

volume = "29",

pages = "175--178",

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publisher = "Centers for Disease Control and Prevention (CDC)",

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TY - JOUR

T1 - Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada

AU - Boodman, Carl

AU - Loomer, Courtney

AU - Dibernardo, Antonia

AU - Hatchette, Todd

AU - LeBlanc, Jason J

AU - Waitt, Brooks

AU - Lindsay, L Robbin

PY - 2023/1

Y1 - 2023/1

N2 - Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%-95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable.

AB - Whole blood is the optimal specimen for anaplasmosis diagnosis but might not be available in all cases. We PCR tested serum samples collected in Canada for Anaplasma serology and found 84.8%-95.8% sensitivity and 2.8 average cycle threshold elevation. Serum can be acceptable for detecting Anaplasma spp. when whole blood is unavailable.

KW - Animals

KW - Humans

KW - Anaplasmosis/diagnosis

KW - Real-Time Polymerase Chain Reaction

KW - Anaplasma phagocytophilum/genetics

KW - Canada/epidemiology

U2 - 10.3201/eid2901.220988

DO - 10.3201/eid2901.220988

M3 - A1: Web of Science-article

C2 - 36573611

SN - 1080-6040

VL - 29

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JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 1

ER -

Using Serum Specimens for Real-Time PCR-Based Diagnosis of Human Granulocytic Anaplasmosis, Canada

Abstract

Keywords

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