What Is the added benefit of oropharyngeal swabs compared to nasal swabs alone for respiratory virus detection in hospitalized children aged

Fatimah S Dawood, Jorge Jara, Dora Estripeaut, Ofelina Vergara, Kathia Luciani, Mary Corro, Tirza de León, Ricardo Saldaña, Juan Miguel Castillo Baires, Rafael Rauda Flores, Rafael A Cazares, Yarisa Sujey Brizuela de Fuentes, Danilo Franco, Melissa Gaitan, Eileen Schneider, LaShondra Berman, Eduardo Azziz-Baumgartner, Marc-Alain Widdowson

Research output: Contribution to journalA1: Web of Science-articlepeer-review

Abstract

We evaluated the added value of collecting both nasal and oropharyngeal swabs, compared with collection of nasal swabs alone, for detection of common respiratory viruses by reverse transcription-polymerase chain reaction in hospitalized children aged <10 years. Nasal swabs had equal or greater sensitivity than oropharyngeal swabs for detection of respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, and influenza virus but not parainfluenza virus. The addition of an oropharyngeal swab, compared with use of a nasal swab alone, increased the frequency of detection of each respiratory virus by no more than 10% in children aged <10 years.

Original languageEnglish
JournalJournal of Infectious Diseases
Volume212
Issue number10
Pages (from-to)1600-3
Number of pages4
ISSN0022-1899
DOIs
Publication statusPublished - 2015

Keywords

  • Child
  • Child, Hospitalized
  • Child, Preschool
  • Humans
  • Infant
  • Infant, Newborn
  • Molecular Diagnostic Techniques
  • Mouth/virology
  • Nasal Cavity/virology
  • Prospective Studies
  • Respiratory Tract Infections/diagnosis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Specimen Handling/methods
  • Virus Diseases/diagnosis
  • Viruses/isolation & purification

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