Whole-genome sequencing of Theileria parva strains provides insight into parasite migration and diversification in the African continent

K. Hayashida, T. Abe, W. Weir, R. Nakao, K. Ito, K. Kajino, Y. Suzuki, F. Jongejan, D. Geysen, C. Sugimoto

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    Abstract

    The disease caused by the apicomplexan protozoan parasite Theileria parva, known as East Coast fever or Corridor disease, is one of the most serious cattle diseases in Eastern, Central, and Southern Africa. We performed whole-genome sequencing of nine T. parva strains, including one of the vaccine strains (Kiambu 5), field isolates from Zambia, Uganda, Tanzania, or Rwanda, and two buffalo-derived strains. Comparison with the reference Muguga genome sequence revealed 34 814-121 545 single nucleotide polymorphisms (SNPs) that were more abundant in buffalo-derived strains. High-resolution phylogenetic trees were constructed with selected informative SNPs that allowed the investigation of possible complex recombination events among ancestors of the extant strains. We further analysed the dN/dS ratio (non-synonymous substitutions per non-synonymous site divided by synonymous substitutions per synonymous site) for 4011 coding genes to estimate potential selective pressure. Genes under possible positive selection were identified that may, in turn, assist in the identification of immunogenic proteins or vaccine candidates. This study elucidated the phylogeny of T. parva strains based on genome-wide SNPs analysis with prediction of possible past recombination events, providing insight into the migration, diversification, and evolution of this parasite species in the African continent.
    Original languageEnglish
    JournalDNA Research
    Volume20
    Issue number3
    Pages (from-to)209-220
    Number of pages12
    ISSN1340-2838
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Animal diseases
    • East Coast fever
    • Theileriasis
    • Theileria parva
    • Cattle
    • Isolation
    • Comparison
    • Genetic diversity
    • Phylogeny
    • Nucleotide sequences
    • Polymorphism
    • Strains
    • Classification and regression trees
    • Immunogenetics
    • Recombination
    • Migration
    • Evolution
    • Zambia
    • Africa-Southern

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