Diagnosis of trypanosomatid infections: targeting the spliced leader RNA

Pablo González-Andrade, Mamady Camara, Hamidou Ilboudo, Bruno Bucheton, Vincent Jamonneau, Stijn Deborggraeve

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

18 Downloads (Pure)

Uittreksel

Trypanosomatids transcribe their genes in large polycistronic clusters that are further processed into mature mRNA molecules by trans-splicing. During this maturation process, a conserved spliced leader RNA (SL-RNA) sequence of 39 bp is physically linked to the 5' end of the pre-mRNA molecules. Trypanosomatid infections cause a series of devastating diseases in man (sleeping sickness, leishmaniasis, Chagas disease) and animals (nagana, surra, dourine). Here, we investigated the SL-RNA molecule for its diagnostic potential using reverse transcription followed by real-time PCR. As a model, we used Trypanosoma brucei gambiense, which causes sleeping sickness in west and central Africa. We showed that the copy number of the SL-RNA molecule in one single parasitic cell is at least 8600. We observed a lower detection limit of the SL-RNA assay in spiked blood samples of 100 trypanosomes per milliliter of blood. We also proved that we can detect the trypanosome's SL-RNA in the blood of sleeping sickness patients with a sensitivity of 92% (95% CI, 78%-97%) and a specificity of 96% (95% CI, 86%-99%). The SL-RNA is thus an attractive new molecular target for next-generation diagnostics in diseases caused by trypanosomatids.

TaalEngels
TijdschriftJournal of Molecular Diagnostics
Volume16
Exemplaarnummer4
Pagina's (van-tot)400-404
Aantal pagina's5
ISSN1525-1578
DOI's
StatusGepubliceerd - 2014

Trefwoorden

  • Humans
  • Limit of Detection
  • RNA, Protozoan
  • RNA, Spliced Leader
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcription
  • Trypanosoma brucei gambiense
  • Trypanosomiasis, African

Vingerafdruk Bekijk de onderzoeksthema's van 'Diagnosis of trypanosomatid infections: targeting the spliced leader RNA'. Samen vormen ze een unieke vingerafdruk.

Dit citeren